蠲痹汤加减对胶原诱导性关节炎大鼠作用机制OA

Objective:To investigate mechanism of action of modified Juanbi decoction on type Ⅱ collagen-in-duced arthritis(CIA)in rats.Methods:Thirty SPF-grade SD rats were selected and randomly divided into blank con-trol group,model group,tripterygium glycosides group,and two dosage groups of modified Juanbi decoction(high,medium,and low doses).Each group consisted of 6 rats.The CIA model was established by inducing with bovine type Ⅱ collagen and Ficoll complete adjuvant emulsion.After successful modeling,the rats were administered intra-gastrically once daily for 4 weeks.The swelling degree of the ankle joint was measured dynamically;after the last ad-ministration,the blood rheological parameters such as whole blood viscosity,erythrocyte aggregation index,and erythrocyte sedimentation rate were detected;the wet weights of the spleen and thymus were weighed,and the organ coefficients were calculated;the peripheral blood T lymphocyte subsets(CD3+,CD4+,CD8+)were detected by flow cytometry;the serum levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)were detected by ELISA;the pathological changes of the ankle joint synovium and cartilage tissue were observed by HE staining.Results:Com-pared with the model group,modified Juanbi decoction significantly reduced the width and thickness of the ankle joint swelling in CIA rats(P＜0.05),improved the pathological damage such as synovial hyperplasia and inflammatory cell infiltration;significantly reduced the whole blood viscosity,erythrocyte aggregation index,and erythrocyte sedi-mentation rate at high,medium,and low shear rates(P＜0.05);inhibited the enlargement of the spleen and thymus(P＜0.05);significantly adjusted the CD4+/CD8+ratio and downregulated the serum levels of TNF-α and IL-1β(P＜0.05).Conclusion:This study,based on the"blood-activation-immune-inflammation"three-dimensional regula-tory network,reveals the"strengthening the body,detoxifying,and promoting circulation to relieve arthralgia"effect of modified Juanbi decoction,and explaining mechanism of modified Juanbi decoction in the treatment of rheumatoid arthritis.