鲜铁皮石斛不同工艺提取物的药效学比较OA

Objective To compare the pharmacological effects of extracts of fresh Dendrobium officinale Kimura&Migo(D.officinale)via two processes(freshly squeezed,freshly squeezed reflux)and the extract from dried D.officinale(prepared by reflux process),screen the optimal preparation technology,and promote the popularization and application of fresh herb preparation.Methods Three distinct extracts of D.officinale were prepared:freshly squeezed extract,freshly squeezed reflux extract,and dried product reflux extract,with three dosage levels:high(8 g·kg-1),medium(4 g·kg-1),and low(2 g·kg-1).The differences in pharmacological effects among these extracts were evaluated using four animal models in mice:ethanol-induced gastric ulcer,constipation,atropine-induced gastrointestinal motility disorder,and acetic acid-induced writhing pain.Results All three extracts alleviated ethanol-induced gastric ulcers,with the ulcer index of drug-treated groups significantly lower than that of model group(P<0.05 or P<0.01).The ulcer index in high-dose freshly squeezed group was significantly better than that in high-dose dried product group(P<0.05).In the defecation experiment with constipated mice(due to dryness and stagnation),the laxative effects of the three extracts showed a certain dose related trend,the number and weight of feces in high-dose freshly squeezed group were significantly higher than those in model group(P<0.05);In the gastrointestinal motility disorder model,only the freshly squeezed concentration extract could promote gastric emptying in mice(P<0.05).Notably,none of the three extracts of D.officinale showed significant analgesic effect.Conclusion The pharmacological effects of fresh D.officinale are superior to those of the dried product.Among the tested extraction processes,the freshly squeezed method yields the optimal effects in anti-gastric ulcer and promoting gastrointestinal motility.This finding provides the optimal extraction process for exerting the pharmacological effects of fresh D.officinale,thereby facilitating its further popularization and application.