基于数据挖掘和网络毒理学探究苦参碱诱发癫痫发作的作用机制OA

Objective To explore the potential mechanism of matrine-induced epileptic seizures and provide a theoreti-cal basis for its neurotoxicity research.Methods Based on data mining and network toxicology methods,the targets related to matrine and epilepsy were integrated,and the enrichment analysis of their intersection targets was conducted.Further,mul-tiple methods such as protein-protein interaction networks and machine learning were combined to screen core toxicological biomarkers.Molecular docking was utilized to verify the binding activity of matrine with the biomarkers.Results A total of 63 potential targets of matrine-induced epilepsy were screened out.Enrichment analysis indicated that they mainly participa-ted in key molecular pathways such as ferroptosis,JAK-STAT signaling pathway,and IL-17 signaling pathway.Eventually,five core toxicological biomarkers(SQSTM1,NFE2L2,JAK2,Akt1,BCL2)were determined.Meanwhile,the molecular docking results showed that matrine had good binding activity with the core targets.Conclusion Matrine may induce epi-leptic seizures by acting on core targets such as SQSTM1,NFE2L2,and JAK2,and regulating multiple pathways including ferroptosis,JAK-STAT,and IL-17 signaling pathways in a coordinated manner.This study provides new ideas and theoretical basis for clarifying the neurotoxic mechanism of matrine and potential intervention strategies.