青藤碱对大鼠肺动脉高压的治疗作用及其关键信号通路分析OA

Objective To explore the preventive and therapeutic effects of sinomenine(SIN)on monocrotaline(MCT)-induced pulmonary arterial hypertension(PAH)in rats,as well as its potential regulatory signaling pathways.Methods A rat model of PAH was established via MCT induction.Right heart catheterization was performed to measure the right ventricular systolic pressure(RVSP)in rats.High-throughput sequencing technology was used to analyze the gene expression profile of rat lung tissues,and enrichment analysis was employed to screen the key signaling pathways through which SIN ameliorates MCT-induced PAH in rats.Finally,quantitative real-time polymerase chain reaction(qRT-PCR)was utilized to detect the expression of core genes in the identified pathways.Results SIN significantly inhibited the MCT-induced increase in RVSP and thickness of pulmonary arterial vessels in rats.Analysis of lung tissue transcriptome data and detection of pulmonary arterial vascular genes revealed that SIN might ameliorate MCT-induced PAH in rats by inhibiting the extracellular matrix(ECM)-receptor interaction signaling pathway.Conclusion SIN may ameliorate MCT-induced PAH in rats by inhibiting the ECM-receptor interaction signaling pathway.