超高效液相色谱-串联质谱法同时测定红花中甜菜安、甜菜宁及乙氧呋草黄残留的分析方法研究OA

This study aimed to establish a rapid,sensitive,and accurate method using Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry(UPLC-MS/MS)for the simultaneous determination of the carbamate herbicides desmedipham and phenmedipham,and the benzofuran herbicide ethofumesate in fresh and dried safflower(Carthamus tinctorius L.).The samples were extracted with 0.1％formic acid-acetonitrile solution,followed by liquid-liquid partitioning induced by sodium chloride.The upper organic phase was filtered and directly injected for analysis,and quantification was performed using the external standard method.The results showed that all three analytes exhibited good linearity within the concentration ranges of 0.005～0.2 mg/L(in solvent)and 0.01～1 mg/L(in matrix),with correlation coefficients(R)greater than 0.9970.At fortification levels of 0.02,0.1,and 1 mg/kg,the average recoveries ranged from 88％to 110％,with relative standard deviations(RSDs)of 1.5％and 12.3％.The limits of quantification(LOQs)for all analytes were 0.02 mg/kg,and the limits of detection(LODs)were at the 10-2 ng level.Matrix effect evaluation revealed significant signal suppression in both fresh and dried safflower matrices,ranging from-42.4％to-91.3％.This method is simple,reproducible,and highly sensitive,meeting the requirements for rapid screening and confirmatory analysis of desmedipham,phenmedipham,and ethofumesate residues in safflower,and providing an effective technical tool for quality and safety supervision of related products.