六盘山药用桑黄分离鉴定与培养研究OA

[Objective]Relying on the high-quality mountain ecological resources of Liupanshan,this study excavated Sanghuang spp.germplasm resources,systematically investigated the biological characteristics of the target strain,and screend the optimal culture conditions for mycelial growth.[Method]Using Sanghuang spp.fruiting bodies collected from Liupanshan National Nature Reserve as experimental materials,strain S-1 was obtained and purified via tissue isolation;molecular phylogenetic identification was performed based on ITS sequences,the optimal culture conditions for mycelia were determined through single-factor experiments,and the formulas of three-stage culture media for mother culture,original culture and cultivation culture were optimized synchronously.[Result]Combining morphological observation and molecular identification based on ITS sequences,the isolated strain S-1 was identified as Phellinus igniarius.The optimal carbon source,nitrogen source and inorganic salt for mycelial growth of this strain were sucrose,yeast extract powder and ferric sulfate respectively,and the optimal culture temperature was 25℃.The optimal medium for mother culture was wheat grain substrate,the optimal medium for original culture was miscellaneous sawdust substrate,and the optimal medium for cultivation culture was sawdust and wheat bran composite substrate.[Conclusion]The wild Phellinus igniarius strain S-1 was isolated and purified from Liupanshan,and the optimal culture conditions for mycelial growth and the matching three-stage medium formula of this strain were clarified.This study can provide bacterial strain basis and standardized culture technical reference for artificial domestication,large-scale cultivation and germplasm resource development of Sanghuang spp.in Liupanshan.