不同产区赤霞珠葡萄根际微生物特征与葡萄果实品质相关性研究OA

[Objective]To clarify the association mechanism between the microbial community characteristics in the rhizosphere soil of wine grapes(Cabernet Sauvignon)on the eastern foothills of Helan Mountain and the quality of the berries,and to improve the theory of microbial terroir regulation in the production area.[Method]With 10-year-old Cabernet Sauvignon grapes from 7 representative wineries in the Helan Mountain production area as the research objects,and samples of rhizosphere soil and mature berries were systematically collected.Based on high-throughput sequencing technologies of 16S rRNA and ITS rRNA,the structure and diversity of soil bacterial and fungal communities were analyzed,and core quality indicators such as soluble solids,titratable acid,tannin,total phenols,and anthocyanins were simultaneously determined.Pearson correlation analysis was used to reveal the coupling relationship between the two.[Result]There were significant differences in the α-diversity index(Chao1,Shannon)of rhizosphere microorganisms among different wineries(P＜0.05).The bacterial diversity was the highest in Meihe Winery(M1),and the lowest in Yuquanying Winery(M4).In terms of berry quality,the soluble solids(29.67 °Brix)and anthocyanin(5.00 mg/g)content in M1 production area were significantly higher than those in other wineries,while the soluble solids content(24.50 °Brix)was the lowest in M4.The titratable acid content generally fluctuated within the range of 5.15-6.59 g/L,with the lowest content in Lilan Winery(M3).The abundance of beneficial bacteria such as Bacillus,Pseudomonas,etc.was significantly positively correlated with the soluble solids,total phenols,and anthocyanin contents of the berries(P＜0.05),while the abundance of fungal groups was negatively correlated with the titratable acid content.At the bacterial phylum level,the Gemmatimonadota,Desulfobacterota,etc.were positively correlated with the quality indicators,while the Verrucomicrobiota,Bacteroidota,etc.were negatively correlated with the quality indicators;at the fungal phylum level,the Ascomycota,Chytridiomycota,etc.were positively correlated with the quality indicators,while the Glomeromycota,Mortierellomycota were negatively correlated with the quality indicators.[Conclusion]The microbial community structure in the rhizosphere of Cabernet Sauvignon on the eastern foothills of Helan Mountain has significant regional specificity,and it can be mediated by regulating soil nutrient cycling to cause berry quality differentiation.The research results can provide support for the exploration of terroir potential in the production area and the improvement of wine quality differentiation.