痛风汤对大鼠湿热蕴结型急性痛风性关节炎模型影响OA
Effect of Tongfeng Decoction(痛风汤)on Rat Model of Acute Gouty Arthritis with Damp Heat Accumulation Type
目的 探讨痛风汤对大鼠湿热蕴结型急性痛风性关节炎模型的抗炎作用机制.方法 将120只SD大鼠(体质量180～200 g,雌雄兼用)随机分组,诱导建立大鼠湿热蕴结型急性痛风性关节炎模型,痛风汤给药干预后测定各实验组大鼠血清及关节液中白细胞介素-1β(IL-1β)、白细胞介素-18(IL-18)和肿瘤坏死因子-α(TNF-α)的含量变化,关节软骨中Toll样受体4(TLR4)、核因子-κB(NF-κB)、NLRP3、IκB 的mRNA 的表达情况以及关节软骨中NLRP3、IκB、NF-κB、TLR4蛋白的表达情况,并将软骨组织HE染色后观察其在细胞学上的改变.结果 大鼠湿热蕴结型急性痛风性关节炎模型诱导成功.与空白组相比,模型组大鼠血清中的IL-1β、IL-18和TNF-α含量显著升高(P<0.05);关节软骨中TLR4、NF-κB、NLRP3 mRNA的表达显著升高(P<0.05),IκB mRNA的表达显著降低(P<0.01);关节软骨中TLR4、NF-κB、NLRP3蛋白的表达水平显著升高,IκB蛋白的表达水平显著降低.与模型组、阳性对照组相比,痛风汤低、中、高剂量组上述指标均有不同程度改善.同时,大鼠关节软骨HE染色结果显示,与模型组比较,痛风汤给药组及阳性组大鼠关节软骨组织结构形态趋于正常化,病理学改变明显改善.结论 痛风汤对大鼠湿热蕴结型急性痛风性关节炎具有显著的抗炎作用.
Objective To investigate the anti-inflammatory mechanism of Tongfeng Decoction(痛风汤)on acute gouty arthritis model with damp heat accumulation type in rats.Methods 120 SD rats(weighing 180-200 g,both male and female)were randomly divided into groups.The rat model of acute gouty arthritis with damp heat accumulation type was induced.After intervention with Tongfeng Decoction,the changes in the levels of IL-1β,IL-18 and TNF-α in the serum and joint fluid of each experimental group were measured.The mRNA expression of TLR4,NF-κB,NLRP3,and IκB in the articular cartilage,as well as the protein expression of NLRP3,IκB,NF-κB,and TLR4 in the articular cartilage were assessed.The articular cartilage was stained with HE to observe the cytological changes.Results The rat model of acute gouty arthritis with damp heat accumulation type was successfully induced.Compared with the control group,the levels of IL-1β,IL-18,and TNF-α in the serum of the model group rats were significantly increased(P<0.05);the mRNA expression of TLR4,NF-κB,and NLRP3 in the articular cartilage was significantly increased(P<0.05),and the expression of IκB mRNA was significantly decreased(P<0.01);the protein expression levels of TLR4,NF-κB,and NLRP3 in the articular cartilage were significantly increased,while the expression level of IκB protein in the articular cartilage of the model group rats was significantly decreased.Compared with the model group and the positive control group,the above indicators in each experimental group showed varying degrees of improvement.Meanwhile,the HE staining results of the rat articular cartilage showed that compared with the model group,the tissue structure and morphology of the articular cartilage in the Tongfeng Decoction group and the positive control group tended to normalize,and the pathology was significantly improved.Conclusion Tongfeng Decoction has a significant anti-inflammatory effect on acute gouty arthritis with damp heat accumulation type in rats.
安玉芳;刘陆;崔立建;周长春;王浩;王春雨
黑龙江省中医医院,黑龙江 哈尔滨 150001黑龙江省中医医院,黑龙江 哈尔滨 150001黑龙江省中医医院,黑龙江 哈尔滨 150001黑龙江省中医医院,黑龙江 哈尔滨 150001黑龙江省中医医院,黑龙江 哈尔滨 150001黑龙江省药品检验研究院,黑龙江 哈尔滨 150001
医药卫生
痛风汤湿热蕴结急性痛风性关节炎
Tongfeng Decoction(痛风汤)damp heat accumulationacute gouty arthritis
《辽宁中医药大学学报》 2026 (8)
11-16,后插1,7
黑龙江省自然科学基金联合引导项目(LH2021H071)
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