LncRNA PVT1/c-Myc表达对弥漫大B细胞淋巴瘤的影响及相关机制分析OA

Objective To investigate the effects of PVT1 on c-Myc expression and phosphorylation in diffuse large B-cell lymphoma(DLBCL)and its role in tumorigenesis and progression.Methods Immunohistochemistry and RT-qPCR were used to evaluate PVT1,c-Myc,and Ki67 expression in DLBCL tissues and reactive hyperplastic lymph nodes(RHLNs),and the correlation between PVT1 and c-Myc expression was analyzed.RT-qPCR and Western blot-ting were used to measure the mRNA or protein expression of PVT1,c-Myc,PCNA,p65,phospho-c-Myc(Thr58),and phospho-c-Myc(Ser62)in DLBCL cell models.CCK-8 assays and flow cytometry were used to determine the ef-fects of aberrant PVT1 expression on DLBCL cell proliferation,cell-cycle progression,and apoptosis.Results PVT1 and c-Myc expression levels were significantly higher in DLBCL tissues than in RHLNs,and their expression levels were positively correlated(P<0.001,r=0.782).Both were associated with clinical stage and Ki67 expression(P<0.05).PVT1 overexpression significantly increased c-Myc,p65,and PCNA expression in DLBCL cells,whereas PVT1 knockdown had the opposite effects.PVT1 overexpression significantly decreased phospho-c-Myc(Thr58)protein expression,whereas PVT1 knockdown produced the opposite trend(P<0.05).Conclusion PVT1 and c-Myc expression levels are positively correlated in DLBCL,and their overexpression is associated with poor prog-nosis and tumor progression.PVT1 may promote DLBCL tumorigenesis and progression by upregulating c-Myc expres-sion,suggesting that PVT1 may be a potential target for DLBCL treatment and prognostic assessment.