紫杉醇-薏苡仁油-β-榄香烯脂质微乳复合递药系统的制备及体外释药研究OA
Preparation and in vitro drug release investigation of paclitaxel-coix seed oil-β-elemene lipid microemulsion composite drug delivery system
基于前期成功制备的紫杉醇-薏苡仁油微乳,进一步将其包封于β-榄香烯脂质层中,构建了紫杉醇-薏苡仁油-β-榄香烯脂质微乳复合递药系统,并对其进行了系统表征及药物含量测定.动态光散射结果显示,紫杉醇-薏苡仁油-β-榄香烯脂质微乳复合物的粒径为(128.10±2.86)nm,Zeta电位为(-15.07±1.03)mV.通过透射电子显微镜观察发现,微乳被成功包封于脂质体中,形成了"大球包小球"结构.高效液相色谱法测定结果显示,紫杉醇和β-榄香烯的包封率分别为(99.40±0.51)％和(76.55±0.61)％,载药量分别为(0.90±0.01)％和(3.65±0.02)％.体外释药研究表明,与紫杉醇相比,β-榄香烯具有优先释放特性,这有助于在紫杉醇大量释放前改善耐药微环境.
Based on the previously successfully prepared paclitaxel-coix seed oil microemulsion,it was further encapsulated into the β-elemene lipid layer to construct a paclitaxel-coix seed oil-β-elemene lipid microemulsion composite drug delivery system,which was systematically characterized and its drug content was determined.Dynamic light scattering(DLS)results showed that the particle size of the composite system was(128.10±2.86)nm,and the Zeta potential was(-15.07±1.03)mV.Transmission electron microscopy(TEM)observation revealed that the microemulsion was successfully encapsulated within the liposomes,forming a"larger sphere encapsulating small sphere"structure.High performance liquid chromatography(HPLC)showed that the encapsulation efficiencies of paclitaxel and β-elemene were(99.40±0.51)％and(76.55±0.61)％,respectively,and the drug loading capacities were(0.90±0.01)％and(3.65±0.02)％,respectively.In vitro drug release experiments showed that β-elemene exhibited preferential release compared with paclitaxel,which may help ameliorate in the drug-resistant microenvironment before the massive release of paclitaxel.
熊锐;曹雨庆;张紫薇;潘青松;钱智磊;陈云艳
皖南医科大学药学院,安徽芜湖皖南医科大学药学院,安徽芜湖皖南医科大学药学院,安徽芜湖皖南医科大学药学院,安徽芜湖南京医科大学附属脑科医院,江苏南京皖南医科大学药学院,安徽芜湖
医药卫生
紫杉醇β-榄香烯微乳脂质体
paclitaxelβ-elemenemicroemulsionliposome
《杭州师范大学学报（自然科学版）》 2026 (4)
401-407,7
国家自然科学基金项目(82404747)安徽省自然科学基金项目(2108085QH370)安徽省优秀青年教师培育项目(YQYB2024046)皖南医学院校重点科研基金项目(WK2024ZZD11)安徽省教育厅自然科学研究项目(2025AHGXZK30634)
评论