特征图谱联合薄层色谱鉴别拳参标准汤剂、配方颗粒及易混品OA

Objective:To establish methods for thin-layer chromatography(TLC)and quantitative characteristic chromatogram to provide a scientific basis for the identification and quality control of Quanshen(Polygonum Bistorta L)standard decoction and its adulterants.Methods:TLC was employed to identify the Quanshen(Polygonum Bistorta L.)standard decoction,formula granules,and its adulterants,using Quanshen(Polygonum Bistorta L.)reference herbs,gallic acid and chlorogenic acid as controls.Additionally,ultra-performance liquid chromatog-raphy(UPLC)was used to establish a quantitative characteristic chromatogram of Quanshen(Polygonum Bistorta L.).The characteristic chromatograms were analyzed using similarity evaluation software,cluster analysis(CA),principal component analysis(PCA)and orthogonal partial least square discriminant analysis(OPLS-DA)to identify the main differences between Quanshen(Polygonum Bistorta L.)and its adulterants.The differential components were quantitatively determined.Results:TLC revealed distinct fluorescence spots between the standard decoction of Quanshen(Polygonum Bistorta L)and its adulterants.The similarity evaluation software analyzed the characteristic chromatograms of 15 batches of Quanshen(Polygonum Bistorta L.)standard decoction,establishing a reference chromatogram.The similarity of Quanshen(Polygonum Bistorta L.)samples was greater than 0.90,while the similarity of adulterants(such as Polygonum viviparum L.and Polygonum paleaceum Wall.)was less than 0.90.CA,PCA and PLS-DA identified gallic acid and chlorogenic acid as the primary differential components between Quanshen(Polygonum Bistorta L.)and its adulterants.Quantitative results showed significant differences in the content of these two components.Conclusion:The established TLC and liquid chromatography methods can quickly,accurately and objectively identify Quanshen(Polygonum Bistorta L.)and its adulterants,significantly improving the accuracy of clinical drug use.