基于SIRT1/Nrf2/HO-1通路探讨姜黄素对乙醇诱导的急性胃黏膜损伤的保护作用及机制OA
The Protective Effect and Underlying Mechanism of Curcumin against Ethanol-Induced Acute Gastric Mucosal Injury via the SIRT1/Nrf2/HO-1 Signaling Pathway
目的:探讨姜黄素对乙醇诱导的急性胃黏膜损伤的保护作用及机制.方法:50只SPF级昆明小鼠随机分为空白组(NC组)、模型组(MD组)、姜黄素低剂量组(Curcumin-L组,50 mg/kg)、姜黄素中剂量组(Curcumin-M组,100 mg/kg)和姜黄素高剂量组(Curcumin-H组,200 mg/kg),每组10只.构建乙醇诱导的急性胃黏膜损伤动物模型,分别予不同干预.通过HE染色观察小鼠胃黏膜组织的病理形态学改变,采用酶联免疫吸附试验(ELISA)检测小鼠血清胃肠激素、炎症因子及氧化应激因子的表达水平,通过免疫组化法测定PCNA的表达水平,通过蛋白质印迹法(Western blotting)检测胃黏膜中SIRT1、Nrf2和HO-1蛋白的表达水平.结果:姜黄素可以显著降低小鼠损伤发生率和胃黏膜损伤评分(P＜0.01),增加小鼠血清Ghrelin水平(P＜0.05),降低MTL、5-HT水平(P＜0.05),降低促炎因子IL-6、IL-1β、TNF-α和MDA的含量(P＜0.05),提高SOD、GSH-Px活性以及PCNA的表达水平(P＜0.05).此外,姜黄素还能够显著提升胃黏膜组织中SIRT1、Nrf2和HO-1蛋白的表达水平(P＜0.05).结论:姜黄素可通过激活SIRT1/Nrf2/HO-1通路,改善乙醇诱导的急性胃黏膜损伤.
Objective:To investigate the protective effect and underlying molecular mechanism of curcumin against ethanol-induced acute gastric mucosal injury.Methods:Fifty SPF-grade Kunming mice were randomly assigned to five groups:blank control group(NC),model group(MD),low-dose curcumin group(Curcumin-L,50 mg/kg),medium-dose curcumin group(Curcumin-M,100mg/kg),and high-dose curcumin group(Curcumin-H,200 mg/kg),with 10 mice per group.An animal model of ethanol-triggered acute gastric mucosal injury was constructed,and corresponding interventions were implemented.Hematoxylin-eosin(HE)staining was performed to observe histopathological lesions in gastric mucosal tissues.Enzyme-linked immunosorbent assay(ELISA)was adopted to measure serum levels of gastrointestinal hormones,inflammatory mediators and oxidative stress markers.Immunohistochemistry was used to detect proliferating cell nuclear antigen(PCNA)expression.Western blotting was conducted to quantify the protein abundances of SIRT1,Nrf2 and HO-1 in gastric mucosa.Results:Curcumin markedly lowered the injury incidence and gastric mucosal lesion score in mice(P＜0.01),elevated serum Ghrelin concentrations(P＜0.05),and downregulated serum MTL and 5-HT levels(P＜0.05).It also decreased the contents of pro-inflammatory cytokines(IL-6,IL-1 β,TNF-α)and malondialdehyde(MDA)(P＜0.05),while enhancing the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),together with PCNA expression(P＜0.05).Moreover,curcumin markedly upregulated the protein expression of SIRT1,Nrf2 and HO-1 within gastric mucosal tissues(P＜0.05).Conclusion:Curcumin ameliorates ethanol-induced acute gastric mucosal injury through activation of the SIRT1/Nrf2/HO-1 signaling pathway.
朱莉;田静;程桂青;郭慧
山东第一医科大学第二附属医院,山东 泰安 271000山东第一医科大学第二附属医院,山东 泰安 271000山东第一医科大学第二附属医院,山东 泰安 271000山东第一医科大学第二附属医院,山东 泰安 271000
医药卫生
急性胃黏膜损伤姜黄素SIRT1/Nrf2/HO-1通路小鼠
acute gastric mucosal injurycurcuminSIRT1/Nrf2/HO-1 signaling pathwaymice
《中医药导报》 2026 (7)
36-41,6
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