石榴皮多酚软膏调节GATA6及相关因子抗痤疮机制OA

Objective:To investigate the anti-acne mechanism of pomegranate peel polyphenols ointment by observing their effects on sebaceous gland spots and serum levels of transcription factor GATA6,transforming growth factor-β(TGF-β),and interleukin-10(IL-10)in golden hamsters.Methods:Totally 36 golden hamsters were randomly divided into six groups[blank group,vehicle group(1 g ointment base),low-dose group(0.480％pomegranate peel polyphenols ointment),medium-dose group(0.960％pomegranate peel polyphenols ointment),high-dose group(1.920％pomegranate peel polyphenols ointment),and positive control group(0.025％Isotretinol yogurt cream)].After 4 weeks of treatment,therapeutic effects were evaluated.Histopathological changes in sebaceous glands were assessed by Hematoxylin-Eosin(HE)staining.Serum IL-10,TGF-β,and TGF-β2 were quantified by enzyme linked immunosorbent assay(ELISA),and cutaneous GATA6 expression was measured by Western blotting.Results:HE staining results showed that pomegranate peel polyphenols ointment reduced sebaceous gland volume,glandular lobes and mature sebocytes in a dose-dependent manner,and the efficacy of high-dose group was similar to positive group.The vehicle group showed lower IL-10 level than blank group,while higher TGF-β1 and TGF-β2 level than blank group(P＜0.05).All treatment groups reversed the above abnormal indexes,increased IL-10 and decreased TGF-β1 and TGF-β2(P＜0.05),with obvious dose-dependent effect.The high-dose group presented the best therapeutic effect,while low-dose group and medium-dose group showed mild efficacy without significant inter-group difference(P＞0.05).The GATA6 expression in vehicle group was lower than that of blank group(P＜0.05).All treatment groups significantly up-regulated GATA6 expression(P＜0.05),and there was no statistical difference between high-dose group and positive control group(P＞0.05).Con-clusion:Pomegranate peel polyphenols ointment may exert its anti-acne effects by regulating GATA6 and its related factors,thereby influencing the secretion of inflammatory cytokines,preventing abnormal keratinization,and so on.