骶尾部中药溻渍抑制MAPK通路介导抗炎抗氧化作用缓解肛肠术后炎症的机制研究OA

Objective:To investigate the mechanism of sacrococcygeal herbal application in reducing postoperative inflammation in anorectal surgery using network pharmacology and animal experiment.Methods:Active ingredients and targets were identified via TCMSP,while disease-related targets were obtained from GeneCards,DisGeNET,OMIM,PharmGkb,and TTD databases.A drug-disease network was constructed,and GO/KEGG enrichment analysis was performed.We established mouse models of post-anorectal surgery,and then measured organ indices,histopathological changes,serum inflammatory factors(hs-CRP,TNF—α,IL-6,IL-17),oxidative stress indicators(T-AOC,MDA),and the expression of MAPK pathway proteins.Results:Network analysis revealed 129 bioactive components and 148 therapeutic targets.The core active components comprise quercetin,daidzein,apigenin,kaempferol,and emodin.The key target genes include MAPK1,MAPK3,TNF,IL-6,and others,and these components are mainly enriched in signaling pathways such as the MAPK and IL-17 pathways.In vivo,the Tazifang(溻渍方)reduced inflammatory scores,decreased hs-CRP,TNF-α,IL-6,IL-17,MDA levels,enhanced T-AOC,and suppressed protein expression of ERK and p38.The high-dose group showed the best effect(P＜0.05).Conclusion:The herbal application may alleviate postoperative inflammatory responses after anorectal surgery by regulating the MAPK signaling pathway,reducing the expression of inflam-matory cytokines such as hs-CRP,TNF-α,IL-6,and IL-17,thereby exerting dual anti-inflammatory and antioxidant effects.