水飞蓟素通过增强抗氧化能力改善湖羊精液冷冻保存效果的研究OA

In order to explore the effects of silymarin(SL)on the cryopreservation of semen of Hu sheep,three 2-3 year old Hu sheep with similar body weight and good body condition were selected as the research objects,and the semen was collected three times every other day.The qualified semen was mixed and diluted with the frozen diluent containing different concentrations of SL.In the control group,no SL was added to the semen,while in the experimental groups,12.5,25,50,100,and 200 μmol/L of SL were added,respectively.After thawing,sperm motility(TM),motility(PM),motility performance(linear velocity[VSL],curvilinear velocity[VCL],path velocity[VAP]and beat frequency[BCF]),plasma membrane integrity rate,acrosome integrity rate,malondialdehyde(MDA)content,superoxide dismutase(SOD)activity,catalase(CAT)activity,mitochondrial membrane potential(MMP)and reactive oxygen species(ROS)level were measured.The results showed that compared with the control group,the sperm motility,motility,exercise performance,plasma membrane integrity rate,acrosome integrity rate,mitochondrial membrane potential level,activities of CAT and SOD(P<0.05)were significantly increased in the 50 μmol/L group,while the levels of MDA and ROS(P<0.05)were significantly decreased.The results indicated that 50 μmol/L SL could reduce freezing damage by enhancing the antioxidant capacity of sperm.