绞股蓝皂苷对沂蒙黑猪精液冷冻保存效果的影响OA

In order to explore the effects of different concentrations of Gynostemma pentaphyllum saponins(GPS)on the preservation of frozen semen of Yimeng black pigs,the semen of 12 Yimeng black pigs were mixed and randomly divided into the control group,low dose group(GL group),middle dose group(GM group)and high dose group(GH group).The control group was not added with GPS,and the other three groups were added with GPS at a final mass concentration of 0.2,0.5,and 1 g/L,respectively.The sperm motility,sperm linear velocity(VSL),sperm average path velocity(VAP),sperm linear(LIN),sperm forward(STR),sperm wobble(WOB),plasma membrane damage rate,DNA damage rate,total antioxidant capacity(T-AOC),malondialdehyde(MDA)content,superoxide dismutase(SOD)activity,mitochondrial membrane potential,and reactive oxygen species(ROS)level were measured after semen thawing.The relative expression levels of HK2,SOD1 and BCL-2 mRNA were detected.The results showed that compared with the control group,the sperm motility,VSL,VAP,LIN,STR,WOB,mitochondrial membrane potential,SOD activity,T-AOC,and the relative expression of HK2 and BCL-2 mRNA in GL group and CM group were significantly increased(P<0.01),and the relative expression of SOD1 mRNA was significantly increased(P<0.05 or P<0.01).The levels of ROS,plasma membrane damage rate,DNA damage rate and MDA concentration were significantly or extremely significantly decreased(P<0.05 or P<0.01).The sperm motility,VSL,VAP,LIN,STR,mitochondrial membrane potential level,SOD activity,T-AOC,SOD1,HK2 gene mRNA relative expression in GH group were significantly decreased(P<0.01);ROS level,plasma membrane damage rate,DNA damage rate and MDA concentration were significantly increased(P<0.01).It indicated that GPS had a significant dose-dependent effect on the improvement of semen quality of Yimeng black pigs in a certain concentration range.