垂盆草-白英提取工艺优选及活性成分含量测定研究OA

The water decoction of the Sedum sarmentosum-Solanum lyratum herb pair originates from the ancient prescription Qianjia Miaofang and is used to treat lung cancer.To improve the extraction efficiency of its active components,this study compared and optimized the water and ethanol extraction processes using the reflux extraction method.With the extraction yield as the preliminary evaluation index,based on single-factor experiments,a high performance liquid chromatography(HPLC)method was established to accurately determine and compare the content of the flavonoid rutin in the extracts,in order to investigate the extraction effect of different processes on anti-tumor active components.The results showed that the optimal conditions for water extraction were a solid-liquid ratio of 1∶10 and extracting at 90℃for 40 min;the optimal conditions for ethanol extraction were 80%ethanol,a solid-liquid ratio of 1∶10,and extracting at 80℃for 90 min.Under the two methods,the average contents of rutin in the ethanol and water extracts were 1.787 μg/g and 0.482 μg/g,respectively,and the RSD values of all methodological validations were less than 5%.The extraction results of the 80%ethanol reflux process were significantly better than those of the water extraction method,and the content of anti-tumor active components in the ethanol extract was significantly higher than that in the water extract.This study provided an experimental basis for the in-depth development and utilization of the Sedum sarmentosum-Solanum lyratum herb pair.