肠道菌群与阿尔茨海默病双向因果关联的孟德尔随机化法分析OA

Aim:To investigate the potential causal relationship between gut microbiota(GM)and Alzheimer's disease(AD).Methods:Genome-wide association study(GWAS)data of 473 GM variants,as well as GWAS of AD from the Finn-ish R12 database were collected.Using single nucleotide polymorphisms(SNPs)of these 473 GM variants as exposures and AD as the outcome variable,forward Mendelian randomization(MR)analysis was conducted;using AD-associated SNPs as exposures and the microbial communities identified by forward MR analysis as the outcome variable,reverse MR analysis was performed.Results:The results of the inverse variance weighting(IVW)method for forward MR indicated that cya-nobacteria,and Francisellales Firmicutes-CAG-110[OR(95%CI)was 1.241(1.006-1.531)],[1.115(1.003-1.240)],[1.594(1.121-2.266)],Lachnospiraceae-CAD-488,Eubacterium,Klebsiella,Azorhizobium,Pseudocatenibacte-rium sp,GCA-900066135,sp900066135 were positively correlated with AD.There was a negative correlation between AD and sp002160815 species in the order arechlamydiales[OR(95%CI)was 0.586(0.356-0.967)],sporangiales[0.652(0.428-0.993)],family Trichomycteridae-CAG-449,family Desulfovibrio,genus Spirosporium,and genus Maasai.Re-verse MR analysis revealed no reverse causal relationship between the aforementioned 15 GM species and AD.Conclusion:Partial GM may be causal associated with AD.