孔圣枕中丹对糖尿病认知功能障碍小鼠海马组织RAS/RAF/MEK信号通路的影响OA

Aim:To investigate the effects of Kongsheng Zhenzhong Dan(KSZZD)on RAS/RAF/MEK signal pathway of diabetic cognitive dysfunction mice.Methods:Ten db/m mice were selected as the control group,while 30 db/db mice were randomly divided into the model group,the metformin group,and the KSZZD group.The control group and model group received oral administration of 10 mL/(kg·day)of normal saline solution,the metformin group received 200 mg/(kg·day)of metformin aqueous solution,and the KSZZD group received 33.8 g/(kg·day)of KSZZD solution,once daily for 12 weeks.The fasting blood glucose was determined.Subsequently,Morris water maze test was performed to assess the learn-ing and memory abilities;hippocampal tissues were examined via HE and Nissl staining for pathological analysis,neuronal ultrastructure was observed under transmission electron microscopy,RAS and RAF protein expression level as well as MEK phosphorylation level were determined by Western blot,and RAS,RAF,and MEK mRNA expression levels were measured by RT-qPCR.Results:Compared with the model group,both the metformin group and the KSZZD group exhibited reduced fasting blood glucose levels,shortened escape latency,increased number of platform crossings,and prolonged dwell time in target quadrants(P<0.001);the hippocampal neuronal morphology was improved with increased Nissl bodies,relatively in-tact mitochondrial structures,and clear mitochondrial cristae;additionally,the protein expression levels of RAS and RAF,and MEK phosphorylation levels,as well as the mRNA expression levels of RAS and RAF were decreased(P<0.05).Conclu-sion:KSZZD may ameliorate diabetic cognitive dysfunction by inhibiting the RAS/RAF/MEK signaling pathway.