淫羊藿苷对骨质疏松大鼠种植体骨结合及骨相关基因血管内皮生长因子与组织形态学的影响OA

Objective:To investigate the action mechanism of icariin intervention in osteoporotic rats,as well as its effects on implant osseointegration,bone-related genes,vascular endothelial growth factor(VEGF),and histomorphology.Methods:Twenty-six SD clean-grade female rats were selected and ran-domly divided into the sham operation group,the model group,and the icariin group,with 8 rats in each group,excluding 2 rats with failed modeling.The bone parameters around the implants,implant osseointegra-tion,bone tissue morphological changes,bone metabolism index levels,oxidative stress index levels,osteo-blast apoptosis rate,bone-related gene expression,and VEGF expression were compared among the three groups.Results:Compared with the sham operation group,the model group showed significant decreases in the levels of GSH-Px,bone mass in the cancellous bone area,CAT,Tb.Th,osteoprotegerin,osseointegra-tion rate,BMD,25OHD,osteocalcin,Tb.N,OPG,osteopontin,BV/TV,VEGF,and SOD;meanwhile,the levels of ALP,apoptosis rate,Tb.Sp,and MDA were significantly increased(all P<0.05).Compared with the model group,the icariin group exhibited significant increases in the levels of GSH-Px,osseointegra-tion rate,CAT,Tb.N,bone mass in the cancellous bone area,BV/TV,25OHD,osteoprotegerin,Tb.Th,OPG,osteocalcin,BMD,osteopontin,SOD,and VEGF,while the apoptosis rate,MDA,Tb.Sp,and ALP levels were significantly decreased(all P<0.05).Conclusions:Icariin can improve VEGF expression,im-plant osseointegration,and bone-related gene expression in osteoporotic rats,reduce the osteoblast apoptosis rate,and regulate oxidative stress and bone metabolism levels.