谷物酿造料酒非生物浑浊的快速微量测定OA

[Objective]A rapid method to measure the abiotic turbidity of grain-brewed cooking wine was established.[Method]Based on light absorbance of fluids,optical density(OD)at varied wavelengths of light measured by a spectrometer on membrane-filtered supernatant of grain-brewed cooking wine and Formazin standard suspension were obtained with absorbance difference(ΔOD)calculated.The data were correlated with turbidity readings determined by a turbidimeter on same samples,determine the threshold for judging the turbidity of grain-brewed cooking wine.[Result]The transmittance and absorbance measured at a wavelength of 800nm cannot accurately represent the turbidity of the grain-brewed cooking wine,A method to rapidly and accurately measure the turbidity of a grain-brewed cooking wine applying ΔOD at the wavelength of 450nm was established.The measurements significantly correlated with the NTUs obtained by the turbidimeter(R2=0.936).ΔOD450 of 0.024 was set as the threshold for acceptable clarity of cooking wines,with an accuracy rate of greater than 90%.[Conclusion]The newly developed method required small sample size,simple operation,and short testing time.It could be applied for quality control for the industry.