大豆GmFBX1基因的生物信息学及非生物胁迫表达分析OA

F-box proteins are key components of the SCF(SKP1-Cullin1-F-box)ubiquitin ligase complex and extensively participate in plant responses to abiotic stresses.A number of F-box proteins have been proven to regulate stress responses in Arabidopsis thaliana,rice and other plant species.To explore the functions of soybean F-box genes in resistance to abiotic stresses,the F-box gene GmFBX1(Accession No.:Glyma.08G088700)was cloned in this study.Bioinformatics tools and transcriptome sequencing data were comprehensively applied to analyze the basic characteristics of the protein encoded by GmFBX1,coding region structure,regulatory elements in the promoter region,phylogenetic relationships and expression profiles.Quantitative real-time PCR(qRT-PCR)was further used to detect the expression patterns of GmFBX1 under drought and salt stresses.The results showed that the cDNA sequence of soybean GmFBX1 was 1 323 bp in length,encoding a protein of 440 amino acids with an isoelectric point of 8.60 and a molecular weight of 49.651 21 kD.The protein encoded by GmFBX1 contained 46 putative phosphorylation sites.Phylogenetic tree analysis revealed that GmFBX1 had a close genetic relationship with its homologous gene in wild soybean.Subcellular localization assays demonstrated that the GmFBX1-GFP fusion protein was mainly localized in the nucleus.Tissue expression analysis indicated that GmFBX1 exhibited the highest expression level in shoot apical meristems.Both transcriptome data and qRT-PCR results revealed that the expression of GmFBX1 was down-regulated under drought and salt treatments.GmFBX1 may negatively modulate soybean abiotic stress responses through degrading stress-related positive regulators or stabilizing negative regulators.This study provides a theoretical basis for further investigating the role of GmFBX1 in soybean abiotic stress responses.