大豆FBL基因家族鉴定及百粒重相关基因挖掘与单倍型分析OA

F-box proteins are key components of the SCF E3 ubiquitin ligase complex in eukaryotes and are extensively involved in plant growth,development,and stress responses.To investigate the role of the FBL gene family in regulating 100-seed weight in soybean,this study employed bioinformatics methods to identify FBL family genes,analyze their colinearity,physicochemical properties,and conserved motifs,and utilized RNA-seq data to examine the tissue expression patterns and early seed development expression patterns of soybean FBL genes.Four FBL genes specifically expressed during early seed development were selected for qRT-PCR and haplotype analysis to elucidate the structure,evolution,and function of the soybean FBL gene family.The results indicated that 44 soybean FBL genes were identified and named GmFBL1-GmFBL44.The amino acid sequences encoded by GmFBL1-GmFBL44 range from 284 to 650 aa,with isoelectric points ranging from 5.16 to 8.99,and their conserved motifs show high similarity.Intraspecific colinearity analysis revealed 53 duplication events within this gene family,indicating that soybean FBL genes have diverged during evolution.Analysis of gene expression patterns showed that 12 genes in the FBL gene family were specifically expressed in soybean seeds;among them,GmFBL14,GmFBL24,GmFBL25,and GmFBL31 were differentially expressed in the two parental lines during the early stages of seed development;specifically,the expression levels of GmFBL14 and GmFBL25 showed an upward trend,while those of GmFBL24 and GmFBL31 first increased and then decreased.Haplotype analysis identified a single C/G mutation at position-386 bp in GmFBL24,which may be involved in regulating soybean 100-seed weight.These findings provide a theoretical basis for functional studies on the role of the soybean FBL gene family in regulating 100-seed weight.