大豆NF-YA基因家族分析及油分含量相关基因的表达与单倍型分析OA

To comprehensively explore the functions of the soybean NF-YA gene family in soybean growth and development as well as its effects on seed oil content,in this study,21 soybean NF-YA family genes were identified using bioinformatics and molecular biology methods and named GmNFYA1-GmNFYA21.Molecular biological characteristic prediction indicated that NF-YA proteins ranged in length from 205 to 346 amino acids,with molecular weight between 22.87 and 37.91 kD and isoelectric point ranging from 5.73 to 9.98.All were negatively charged hydrophilic proteins,and most NF-YAs(except GmNFYA12,GmNFYA19,and GmNFYA21)were located in the nucleus.Chromosome distribution and collinearity analysis results showed that NF-YA gene family members were distributed on 15 chromosomes,and 28 pairs of genes in this family had collinearity relationships,showing a clear trend of functional differentiation.Gene expression pattern analysis indicated that GmNFYA2,GmNFYA11,GmNFYA14,and GmNFYA17 had expression differences in the EM1 stage of seeds in Suinong 14 and ZYD00006.Haplotype analysis based on 547 soybean germplasms showed that GmNFYA2 and GmNFYA14 had superior haplotypes for oil content.This study provides important clues for comprehensively understanding the biological functions of NF-YA family genes in regulating soybean oil synthesis and metabolism.