豇豆轮纹病拮抗细菌筛选及其生防效果评价OA

Taking the tissues of cowpea plants and the rhizosphere soil as the test materials,the microorganisms were isolated and purified by the dilution plating method.Combined with the plate confrontation culture method,the antagonistic strains were initially screened with the target of the cowpea target spot pathogen.The re-screening was completed by the determination of the broad-spectrum of inhibition,and the control effect of the antagonistic strains on cowpea target spot was evaluated by the detached leaf method,in order to provide a reference for the biological control of cowpea target spot.The results showed that antibacterial rate exceeding 70％against cowpea ring spot.WZ-9 showed an antibacterial rate of 76.57％.After broad-spectrum antifungal activity of WZ-9 revealed that exhibited strong inhibitory activity against all ten tested plant pathogenic fungi.Based on morphological and molecular identification,the strain was identified as Pseudomonas aeruginosa.Further assays indicated that its cell-free fermentation filtrate achieved a 50％inhibition rate against the pathogen.In detached leaf assays,WZ-9 provided a control efficacy of 44.5％against cowpea ring spot.In conclusion,after screening successfully identified P.aeruginosa strain WZ-9,which demonstrates dual functionality by antagonizing various cowpea pathogens and promoting seedling growth.These findings position WZ-9 as a promising candidate for developing new biopesticides.