PEG-6000模拟干旱胁迫对猪苓菌株生长特性的影响OA

Taking three different sources of Polyporus umbellatus strains as the test materials,polyethylene glycol(PEG-6000)was used to simulate drought stress and the effects of different PEG-6000 treatments(T0,T1,T2,T3,T4)on growth indicators such as biomass,osmotic regulation substances,metabolite content,and related enzyme activities in three Polyporus umbellatus strains(S1,S2,S3)were investigated,in order to provide references for the selection of strains and the development and utilization of metabolites in Polyporus umbellatus cultivation under drought stress.The results showed that,1)Low-concentration PEG-6000 stress significantly promoted the growth indicators of Polyporus umbellatus strains.The T2 treatment exhibited increased biomass(3.80 g·L-1),osmotic adjustment substances such as Pro(5.10 μg·g-1),metabolites such as polysaccharides(75.12 mg·g-1),and enzyme activities such as PGM(8.15 U·mg-1)by 23.4％,78.3％,46.6％,and 20.2％compared to the T0 treatment,respectively,with an upward trend as the cultivation time extended.2)High-concentration PEG-6000 stress significantly inhibited some growth indicators of Polyporus umbellatus strains.The T4 treatment showed decreased biomass(2.10 g·L-1),intracellular polysaccharide content(25.10 mg·g-1),and SOD activity(180.12 U·g-1)by 47.5％,37.5％,and 50.2％compared to the T0 group,respectively.3)Correlation analysis revealed a highly significant positive correlation between osmotic adjustment substances such as Pro and soluble protein,metabolites such as MDA,and enzyme activities such as POD,CAT,and PGI with PEG-6000 concentration,while biomass and SOD activity showed a highly significant negative correlation with PEG-6000 concentration.In summary,PEG-6000 stress had a significant bidirectional regulatory effect on the biomass,osmotic adjustment substances,metabolite content,and related enzyme activities of Polyporus umbellatus strains.Low-concentration PEG-6000 promoted metabolite production by enhancing enzyme activities such as PGM,with 150 g·L-1 possibly being its tolerance threshold.