肺形侧耳种质资源主要农艺性状的多样性分析OA

Taking total of 22 Pleurotus pulmonarius strains as the test materials,the range of grades for the measured traits was determined by the least significant difference method and the range method.The genetic diversity was assessed by coefficient of variation,genetic diversity index,and clustering,correlation and principal component analysis,in order to provide effective germplasm resources for the breeding of new varieties.The results showed that 8 agronomic traits such as primordium formation time and pileus length conformed to normal distribution and were divided into three grades by the least significant difference method;while spawn running period and pileus thickness did not conform to normal distribution and were also divided into three grades by the range method.The coefficient of variation of the above 10 agronomic traits among the tested strains ranged from 5.34％to 29.63％.The genetic diversity index of 13 agronomic traits varied from 0.49 to 1.39.Among 13 agronomic traits,the genetic diversity indexes of pileus color,primordium formation time,stipe length,ratio of pileus length to stipe length and stipe shape were relatively large,indicated that the phenotypic diversity of these agronomic traits was relatively abundant.There was a high correlation between primordium formation time and fruiting body maturity time,as well as pileus length and pileus width,stipe diameter and pileus thickness.The clustering analysis showed that all tested strains could be divided into 5 groups.The agronomic traits of cultivated germplasm were highly homogenized,while the agronomic traits of wild germplasm were rich in diversity.Principal component analysis showed the first three principal components accounting for 71.750％of the cumulative contribution,representing pileus characteristics,stipe characteristics and reproduction period,respectively.The comprehensive evaluation of the tested strains was conducted based on principal component analysis.The comprehensive score of the tested strains range was from-1.00 to 1.31.The five strains with higher comprehensive scores can be utilized as available germplasm for breeding new varieties of P.pulmonarius.The phenotypic diversity of P.pulmonarius germplasm resource is abundant,which exhibits significant potential for domestication and utilization.