生育三烯酚富集组分的急性经口毒性和遗传毒性评价OA

OBJECTIVE:To evaluate acute oral toxicity and genotoxicity of tocotrienol-rich fraction(TRF).METHODS:According to the national food safety standards,toxicity grade of TRF was evaluated using an acute oral toxicity test.Genotoxicity was assessed using a bacterial reverse mutation test,a mammalian erythrocyte micronucleus test,and a mouse spermatocyte chromosomal aberration test.RESULTS:The acute oral median lethal dose(LD50)of TRF in mice was greater than 19.4 g/kg,classifying it as non-toxic.In the bacterial reverse mutation test,the number of revertant colonies in two independent experiments(with and without S9 metabolic activation)did not exceed twice the background spontaneous mutation rate,and no dose-response relationship was observed,indicating a negative result.In the mammalian erythrocyte micronucleus test,doses of 2.5,5.0 and 10.0 g/kg TRF did not induce a significant increase in the micronucleus rate of mouse bone marrow polychromatic erythrocytes(P>0.05).In the mouse spermatocyte chromosome aberration test,no significant increases in the chromosome aberration rate or the percentage of aberrant cells were observed at any dose levels(P>0.05).CONCLUSION:Under the conditions of this study,TRF can be classified as non-toxic and it shows no evidence of genotoxicity.