醋鳖甲的毒理学安全性试验研究OA

OBJECTIVE:To study toxicity of vinegar turtle shell.METHODS:Toxicity of vinegar turtle shell was tested according to Procedures and Methods for Toxicological Evaluation of Food Safety(GB 15193).The following tests were conducted:acute oral toxicity test,Ames test,micronucleus test of mouse bone marrow red blood cells,chromosome aberration test of mouse spermatocyte and 28-day oral toxicity test.RESULTS:The half of lethal dose(LD50)values of male and female mice in the acute oral toxicity test were greater than 20.0 g/kg,which was classified as practically non-toxic.The results of Ames test,micronucleus test of mouse bone marrow red blood cells and chromosome aberration test of mouse spermatocyte were all negative.In the 28-day oral toxicity test,no significant changes were observed in total weight gain,total food intake,eye,hematology,blood biochemistry,urine and histopathology of rats in the 3 dose groups of 20,10 and 5 g/kg compared with the control group(P>0.05).CONCLUSION:Under the conditions of this experiment,the vinegar turtle shell showed no acute toxicity or genetic toxicity.It belongs to the non-toxic food raw materials.