Diras2条件性敲除对小鼠食管上皮细胞增殖的影响OA

OBJECTIVE:To provide a new target for early diagnosis and treatment of esophageal cancer,Diras2 conditional knockout mice and control mice were used to investigate control of esophageal epithelial cell proliferation by Diras2.METHODS:Esophagus tissues of Diras2 conditional knockout and control mice were collected.Immunohistochemical(IHC)staining method was used to detect expression of Diras2 in esophageal tissues.HE staining was used to reveal organization forms of esophagus tissues.IHC staining was performed to detect expressions of Ki67,pRaf1,pMEK1/2,pErk1/2 protein in each group.RESULTS:Expression levels of Diras2 protein in the conditional knockout mice were significantly lower than that in control mice(P<0.01).HE staining showed no significant changes in the histomorphology of the esophagus in both groups.Immunohistochemical staining showed that the proportion of Ki67-positive cells in esophageal epithelium of the conditional knockout mice was reduced(P<0.01).At the same time,expressions of pRaf1,pMEK1/2 and pErk1/2 proteins were also significantly reduced(P<0.01).CONCLUSION:Conditional knockout of Diras2 inhibited proliferation of esophageal epithelial cells in mice by regulating the Raf/MEK/Erk signaling pathway.