食管癌中Z-DNA结合蛋白1与细胞焦亡相关分子干扰素调节因子3的相关性及其机制OA

OBJECTIVE:To investigate correlations and underlying mechanisms between Z-DNA binding protein 1(ZBP1)and interferon regulatory factor 3(IRF3),a molecule associated with pyroptosis,in esophageal carcinoma(EC).METHODS:The protein-protein interaction network of ZBP1 and pyroptosis-related molecules was analyzed using the STRING database.Correlations between ZBP1 and pyroptosis-related molecules in EC were assessed via the GEPIA database to screen proteins with the highest correlation coefficient.Immunohistochemistry was used to detect IRF3 expression in EC tissues.A ZBP1 overexpression model was constructed in Eca109 cells,and protein levels of ZBP1 and IRF3 were determined by Western blot.RESULTS:STRING and GEPIA analyses identified that IRF3 had the strongest correlation with ZBP1.Immunohistochemistry showed that IRF3 expression was significantly higher in EC tissues than in adjacent normal tissues(P<0.05),but was not associated with EC prognosis(P>0.05).The ZBP1 overexpression model was successfully verified by markedly increased ZBP1 expression.Western blot demonstrated that IRF3 protein expression was significantly elevated in ZBP1-overexpressing cells compared with the control group(P<0.01),suggesting that upregulated ZBP1 might promote IRF3 expression.CONCLUSION:IRF3 expression was higher in esophageal carcinoma than in normal tissues,and its upregulation may be regulated by ZBP1 in a targeted manner.