环境因素驱动纹彩猎蝽雌虫肠道菌群结构与多样性显著差异OA

The gut bacterial community plays a crucial role throughout the developmental stages of host insects.In this study,based on the PacBio platform and using full-length 16S rDNA amplicon sequencing technology,we systematically analyzed the gut bacterial community characteristics of female Euagoras plagiatus under different living environments.The results revealed that 650 and 239 operational taxonomic units(OTUs)were detected in the guts of wild-type(Y)and laboratory-reared(Z)female E.plagiatus,respectively,with 218 OTUs shared between the two groups.Both Alpha and Beta diversity analyses indicated that the gut bacterial community of wild-type individuals exhibited higher richness and diversity.Furthermore,through bacterial isolation techniques,19 bacterial strains belonging to 6 different species were successfully isolated from the gut of female E.plagiatus.Quantitative real-time PCR(RT-qPCR)analysis further revealed significant differences in the abundance of endogenous bacteria between wild-type and reared insects.This study elucidates the influence of environmental factors on the composition and diversity of the gut bacterial community in female E.plagiatus adults,providing an important theoretical basis for a deeper understanding of their environmental adaptation mechanisms.