马铃薯GARP转录因子家族分析及StARR14-X1基因功能验证OA

[Objective]To identify key genes regulating anthocyanin accumulation in the tubers of colored potatoes(Solanum tuberosum L.),provide genetic resources and technical references for potato molecular breeding.[Method]GARP transcription factors were screened based on transcriptome sequencing data of purple potato tubers at different developmental stages.Bioinformatics analysis was conducted,and functional verification of StARR14-X1 gene related to anthocyanin regulation was performed.[Result]The 12 GARP transcription factors were screened through transcriptome data.The amino acid sequence lengths of 12 GARP transcription factors were concentrated between 148-686 aa,the molecular weight ranged from 16.66 to 75.44 kD,the pI value ranged from 5.13 to 8.05,the fatty index ranged from 71.36 to 97.36,and the instability coefficient ranged from 35.59 to 75.94,all of which belonged to hydrophilic proteins.Subcellular localization prediction showed that 12 GARP transcription factors were distributed in the nucleus,cytoplasm and chloroplasts.The StARR14-X1 gene was successfully transformed into tetraploid tobacco using the Agrobacterium-mediated leaf disc method,and transgenic tobacco plants overexpressing StARR14-X1 were obtained.The anthocyanin contents in the stems and petals of StARR14-X1-overexpressing transgenic lines increased significantly.[Conclusion]StARR14-X1 gene has a positive regulatory function in anthocyanin accumulation and promotes anthocyanin accumulation.