白音华露天矿区不同施肥处理对土壤养分有效性的影响及其微生物响应机理OA

Open-pit coal mining often induces severe soil nutrient depletion,significantly hindering vegetation recovery.In this study,a field experiment was conducted to investigate the effects of different fertilization strategies on soil nutrient dynamics and availability,as well as the mediating role of microbial communities.The treatments included(1)high-,medium-and low-phosphorus(P)doses of inorganic fertilizers,(2)green manure(plant residue),and(3)a microbial fertilizer containing nitrogen-fixing,plant-growth-promoting bacteria and rhizobia.The results demonstrated that,compared to the control,the application of microbial fertilizer significantly increased soil total carbon(C)and total nitrogen(N)contents,rising from 8.47 g·kg−1 to 10.17 g·kg−1 and from 0.37 g·kg−1 to 0.56 g·kg−1,respectively.High-P inorganic fertilizer significantly increased available P content(26.28 mg·kg−1 vs.9.78 mg·kg−1 in the control).Both microbial fertilizer and high-P inorganic fertilizer significantly altered soil fungal community composition,particularly enhancing the relative abundances of Gibberella and Alternaria.These results indicate that both fertilization strategies can improve N and P availability in reclaimed soils by restructuring fungal community composition and thereby modifying soil nutrient status.Overall,the application of microbial fertilizer containing nitrogen-fixing plant-growth-promoting bacteria and rhizobia represents a promising reclamation strategy that supports green mining practices and contributes to ecological restoration efforts in mining areas.