加味薏苡附子败酱散对溃疡性结肠炎大鼠肠黏膜微循环障碍改善作用的研究OA

Objective:Explore the improvement effect of the Modified Yiyi Fuzi Baijiang Powder on intestinal mucosal micro-circulatory disturbance in rats with ulcerative colitis(UC)induced by dextran sulfate sodium(DSS).Methods:A total of 60 SPF-grade rats were randomly divided into a blank group,model group,mesalazine group,and low,medium,and high-dose Modified Yiyi Fuzi Baijiang Powder groups.Except for the blank group,rats in all other groups were treated with 3%DSS to establish the UC model.Following successful model establishment,rats in the mesalazine group and each dose of the traditional Chinese medi-cine group received corresponding treatments via gavage,while rats in both the blank and model groups were given an equivalent amount of physiological saline as a control.H&E staining was used to observe the pathological changes of colon tissue in each group.A blood rheology instrument was used to measure hematological parameters.ELISA was employed to detect serum levels of P-selectin,TXB2,and 6-Keto-PGF1α in the rats.Transmission electron microscopy was utilized to examine the ultrastructure of intestinal microvessels,while Masson's trichrome staining assessed the ratio of microvascular collagen fiber-positive area.Immu-nohistochemical methods were used to detect the levels of VEGF in colonic tissue and to measure MVD in the intestinal mucosa.Result:Compared to the blank group,the model group showed significant pathological damage to the colon,with significantly in-creased levels of blood rheological parameters,P-selectin,TXB2,VEGF,and MVD(P<0.05).The expression level of 6-Keto-PGF1α was significantly decreased(P<0.01),microvascular damage was significant,and the ratio of microvascular collagen fiber-positive area was significantly increased(P<0.01).Compared to the model group,each treatment group showed improvement in colonic pathological damage,with decreased levels of blood rheological parameters,P-selectin,TXB2,VEGF,and MVD(P<0.05).The expression level of 6-Keto-PGF1α was increased(P<0.01),microvascular damage was alleviated,and the ratio of mi-crovascular collagen fiber-positive area was significantly reduced(P<0.01).Among the traditional Chinese medicine groups,the high-dose group demonstrated the most significant efficacy.Conclusion:Modified Yiyi Fuzi Baijiang Powder can effectively im-prove intestinal mucosal microcirculatory disturbance in rats with UC.