208份小麦种质的白粉病抗性评价及抗病基因检测OA

[Objective]The composition of main powdery mildew resistance genes in wheat germplasms was analyzed to provide a reference for breeding of wheat varieties with a good resistance to powdery mildew in Guizhou.[Method]The resistance to powdery mildew at the adult-plant stage of 208 wheat germplasms from home and abroad was identified under the condition of natural disease occurrence in field.6 powdery mildew resistance genes(Pm2a,Pm5e,Pm21,Pm38,Pm39 and PmV)were detected by using wheat functional gene liquid chips in 208 wheat varieties(lines).[Result]The proportion of 44 wheat germplasms with a highly resistance to powdery mildew,22 wheat germplasms with a resistance to powdery mildew,35 wheat germplasms with a moderate resistance to powdery mildew,60 wheat germplasms with a susceptibility to powdery mildew and 47 wheat germplasms with a highly susceptibility to powdery mildew accounted for 21.2%,10.6%,16.8%,28.8%and 22.6%in 208 wheat germplasms,respectively.The detected frequency of Pm39,Pm21 and Pm38 was 51.0%,8.2%and 7.7%,separately.The Pm2a,Pm5e and PmV were not detected from 208 whet germplasms.From the perspective of resistance gene composition and distribution,the number of wheat germplasms with a single gene reached 113.The Pm21+Pm38 combination,Pm21+Pm39 combination and Pm38+Pm39 combination were detected in one wheat germplasm,two wheat germplasms and ten wheat germplasms,respectively.[Conclusion]The resistance level of wheat germplasms in southwest wheat region(Guizhou and Sichuan)is higher than that of wheat germplasms in north wheat region and CIMMYT.Except for Pm39 gene,the detection frequency of other 5 powdery mildew resistance genes was low.The Pm21 was only detected in wheat germplasms from southwest wheat region,and the Pm21 detection frequency of wheat germplasms from Guizhou Province is the highest.