基于BSA-seq技术的饲料油菜植株粗蛋白含量QTL定位研究OA

[Objective]The genetic loci and key genes regulating crude protein trait of forage rapeseed plants were analyzed to provide a reference for improvement of crude protein trait of forage rapeseed plants and breeding of high-quality forage rapeseed varieties.[Method]The BSA-seq technology was used to sequence and conduct association analysis on DNA mixed pool samples of parental lines 21SL-239 and 21SL-756 with extreme crude protein differences and F2 segregating population with extreme differences.[Result]A total of 37 regions significantly associated with crude protein content trait were identified within the 50.46-53.02 Mb interval on chromosome C08 of rapeseed.38 functional genes(ZS11C08G057720,ZS11C08G059050,ZS11C08G058760,etc.)related to protein synthesis,amino acid supply,protein degradation,97 functional genes(ZS11C08G058820,ZS11C08G057600,ZS11C08G061500,ZS11C08G061580,ZS11C08G061590,etc.)related to carbohydrate transport and metabolism,energy production and conversion,transcription regulation,etc.and 104 unknown function genes(ZS11C08G058790,ZS11C08G061450,ZS11C08G059590,etc.)were screened by a functional annotation technology.[Conclusion]One QTL locus related to crude protein content of forage rapeseed plants is located,and 239 candidate genes potentially related to crude protein content are screened.