SKA1在肾透明细胞癌中的生物学特征及与临床特征的相关性OA

Objective To investigate the biological characteristics of spindle and kinetochore-associated complex 1(SKA1)in renal clear cell carcinoma and its correlation with clinical features.Methods First,sequencing data from The Cancer Genome At-las(TCGA)and the Genotype-Tissue Expression(GTEx)database were analyzed to determine the differential expression of SKA1 across pan-cancer.Second,clinical postoperative samples of renal clear cell carcinoma,including tumor tissues and adjacent non-tumor tissues,were collected,and quantitative real-time polymerase chain reaction(qRT-PCR)and Western blot were performed to measure the mRNA and protein expression levels of SKA1.Finally,ROC curves,Kaplan-Meier survival curves,and Cox proportional hazards regression analysis were applied to evaluate the diagnostic efficacy and prognostic value of SKA1 in renal clear cell carcinoma,and a protein-protein interaction network of SKA1 was constructed for functional enrichment analysis.Results Upregulated SKA1 ex-hibited significant oncogenic effects at the pan-cancer level.Experimental validation using clinical samples revealed that both the pro-tein and mRNA expression levels of SKA1 were significantly higher in renal clear cell carcinoma tissues compared with adjacent non-tumor tissues(P<0.01).Upregulation of SKA1 promoted malignant progression of renal clear cell carcinoma and was associated with aggressive clinicopathological features.protein-protein interaction and functional enrichment analyses suggested that SKA1 may exert oncogenic effects by accelerating cell cycle and proliferation-related processes through the regulation of mitosis.Conclusion SKA1 is significantly overexpressed in renal clear cell carcinoma.As an oncogene,SKA1 is closely correlated with poor prognosis of renal clear cell carcinoma and shows favorable diagnostic efficacy.