Electrohydrodynamic bioprinting-induced orientation of cell-laden fibrin-alginate hydrogel for highly-aligned skeletal muscle constructsOA
Bioprinting provides an unparalleled tool for engineering living tissue constructs that mimic the structural organization of native skeletal muscles.However,it remains a challenge for existing bioprinting strategies to recapitulate the highly aligned cellular architectures inside skeletal muscles,primarily due to low printing resolution and limited capability for in situ microenvironmental regulation.Here,we propose to employ the electrical force during the electrohydrodynamic(EHD)bioprinting process to induce the in situ orientation of cell-laden fibrin-alginate hydrogel,which provides nanostructural guidance to the encapsulated cells for the formation of highly aligned skeletal muscle constructs.It was observed that the randomly distributed fibrin protofibril aggregates gradually elongated into uniformly aligned nanofibers at the Taylor cone stage as the applied voltage increased to 3 kV.The oriented fibrin nanofibers further direct in situ cellular alignment along the EHD bioprinting trajectory,facilitating the freeform fabrication of parallelly or circumferentially aligned muscle tissue constructs in vitro.The addition of conductive polymers into the fibrin-alginate hydrogel endows the EHD-bioprinted living constructs with muscle-specific conductivity and cellular organization,which promote myotube differentiation and maturation.The resultant aligned and conductive muscle constructs promoted in situ muscle regeneration and restored lost muscle functions at the defect regions in vivo.The presented EHD bioprinting strategy for fibrin-alginate hydrogel provides a versatile and simple platform to freely fabricate conductive,living tissue constructs with designer cellular alignments.
Ayiguli Kasimu;Zijie Meng;Zhennan Qiu;Yabo Zhang;Lang Bai;Xiao Tan;Ziyu Wang;Ruosen Zhao;Qianxi Gao;Hui Zhu;Zhanguo Tong;Wurikaixi Aiyiti;Dichen Li;Jiankang He
State Key Laboratory for Manufacturing Systems Engineering,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of China State Industry-Education Integration Center for Medical Innovations,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of China School of Mechanical Engineering,Xinjiang University,Urumqi 830017,People''s Republic of ChinaState Key Laboratory for Manufacturing Systems Engineering,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of China Frontier Institute of Science and Technology,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of China State Industry-Education Integration Center for Medical Innovations,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of ChinaState Key Laboratory for Manufacturing Systems Engineering,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of China State Industry-Education Integration Center for Medical Innovations,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of ChinaState Key Laboratory for Manufacturing Systems Engineering,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of ChinaThe First Affiliated Hospital of Xi''an Jiaotong University,Xi''an 710061,People''s Republic of ChinaTangdu Hospital,Air Force Medical University,Xi''an 710038,People''s Republic of ChinaState Key Laboratory for Manufacturing Systems Engineering,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of China State Industry-Education Integration Center for Medical Innovations,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of ChinaState Key Laboratory for Manufacturing Systems Engineering,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of China State Industry-Education Integration Center for Medical Innovations,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of ChinaState Key Laboratory for Manufacturing Systems Engineering,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of ChinaState Key Laboratory for Manufacturing Systems Engineering,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of China State Industry-Education Integration Center for Medical Innovations,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of ChinaState Key Laboratory for Manufacturing Systems Engineering,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of ChinaSchool of Mechanical Engineering,Xinjiang University,Urumqi 830017,People''s Republic of ChinaState Key Laboratory for Manufacturing Systems Engineering,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of China State Industry-Education Integration Center for Medical Innovations,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of ChinaState Key Laboratory for Manufacturing Systems Engineering,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of China National Medical Products Administration(NMPA)Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of China State Industry-Education Integration Center for Medical Innovations,Xi''an Jiaotong University,Xi''an 710049,People''s Republic of China
医药卫生
electrohydrodynamic bioprintingin situ fibrin orientationcell alignmentconductive hydrogelmuscle tissue engineering
《International Journal of Extreme Manufacturing》 2026 (3)
P.695-714,20
supported by the National Natural Science Foundation of China(52405325,52125501)the Key Research Project of Shaanxi Province(2024SF2-GJHX-34)the Postdoctoral Fellowship Program of CPSF(GZB20230573)the China Postdoctoral Science Foundation(2024M762577),the Postdoctoral Project of Shaanxi Province(2023BSHYDZZ30)the Open Research Fund of the State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration(2024KB04)the Open Research Fund of the State Key Laboratory for Manufacturing Systems Engineering(sklms2025014)the Fundamental Research Funds for the Central Universities and Open Research Fund of Xinjiang Uygur Autonomous Region Key Laboratory(2025D04009).
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