Mineralized DNA tetrahedron-structured hydrogels:a dual-functional Scaffold for immunomodulation and bone regenerationOA
Clinical limitations of autografts and allografts have driven advances in bone tissue engineering.Emerging biomaterials offer tunable mechanical and bio-regenerative properties for bone reconstruction.DNA hydrogels have attracted increasing attention due to their extracellular matrix–like architecture and excellent cargo-loading capacity.However,their rapid degradation and limited immunomodulatory activity have hindered their long-term efficacy in bone regeneration.To address these limitations,a mineralized tetrahedral framework nucleic acids(tFNAs)hydrogel(Cap-gel)was engineered to integrate early immunoregulation with sustained osteogenic activity.The stable and programmable spatial structure of tFNAs not only promotes macrophage polarization toward the M2 phenotype by presenting immunomodulatory ligands but also serves as a nucleation template for calcium phosphate crystallization,leading to the formation of nano-mineralized structures with controlled morphology.In vitro,Cap-gel promoted osteogenic differentiation via both immune-dependent and independent pathways,while in vivo,it modulated early immune responses and accelerated bone regeneration in a calvarial defect model.In summary,this study introduces a novel tFNA-based mineralized DNA hydrogel system that integrates immunomodulation and osteogenesis,providing a promising strategy for enhanced bone repair in tissue engineering applications.
Lan Yao;Jiafei Sun;Zhiqiang Liu;Jiale Liang;Yun Wang;Ye Chen;Ruiqing Wang;Tao He;Yichen Yang;Yao He;Yunfeng Lin;Taoran Tian
State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan,ChinaStomatology Hospital,School of Stomatology,Zhejiang University School of Medicine,Zhejiang Provincial Clinical Research Center for Oral Diseases,Key Laboratory of Oral Biomedical Research of Zhejiang Province,Cancer Center of Zhejiang University,Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province,Hangzhou,ChinaState Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan,ChinaState Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan,ChinaState Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan,ChinaState Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan,ChinaState Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan,ChinaState Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan,ChinaState Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan,ChinaMacao Translational Medicine Center,Macao University of Science and Technology.Taipa,Macao SAR,ChinaState Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan,China Sichuan Provincial Engineering Research Center of Oral Biomaterials,Chengdu,Sichuan,ChinaState Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan,China Sichuan Provincial Engineering Research Center of Oral Biomaterials,Chengdu,Sichuan,China
医药卫生
mineralized dna hydrogelsextracellular matrix architecturemineralized ttetrahedron structured hydrogelsimmunomodulationimmunomodulatory activitybone regenerationbone reconstructiondna hydrogels
《Bone Research》 2026 (3)
P.846-860,15
supported by the National Natural Science Foundation of China(82201027 and 82370929)Fundamental and Interdisciplinary Disciplines Breakthrough Plan of the Ministry of Education of China(Grant No.JYB2025XDXM610)Young Elite Scientists Sponsorship Programby CAST(YESS20240133)Major Science and Technology Special Project of Sichuan Province(2025ZDZX0131)Leading Scientist Programfor Basic Research of Sichuan Province(2025JDKXJ0001)The Sichuan Science and Technology Program(2024NSFSC1591 Sichuan Province Youth Science and Technology Innovation Team(2022JDTD0021)Health Commission of Sichuan Province Medical Science and Technology Program(24CGZH02)Research and Develop Program,the West China Hospital of Stomatology Sichuan University(RD03202302,RCDWJS2024-1).
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