Biooxidation of aromatic aldehydes by aldehyde dehydrogenase from Sphingobium sp.using synthetic nicotinamide cofactor biomimeticsOA
An aldehyde dehydrogenase SpALDH2 from Sphingobium sp.has been discovered with the unprecedented ability to utilize oxidized nicotinamide cofactor biomimetics(NCBsOX).Among eight NCBsOX tested,3-carbamoyl-1-(4-carboxybenzyl)pyridin-1-ium(p-BANA+)enabled 96.8%conversion of syringaldehyde,comparable to NAD+.SpALDH2 exhibited a kcat/KM of 21.418 mM^(-1) min^(-1) with p-BANA+,nearly 500-fold higher than with other NCBs_(OX).Molecular docking and interaction analyses suggested that hydrogen bonding and pi-pi stacking between the para-carboxyl group of p-BANA+and key active-site residues contribute to its superior compatibility.These results support SpALDH2 as a promising catalyst for NCBsOX-mediated oxidation.
Jieyu Zhou;Xiangyuan Gu;Siyi Chen;Yichun Zhu;Ye Ni
Key laboratory of Industrial Biotechnology,School of Biotechnology,Jiangnan University,Wuxi,214122,ChinaKey laboratory of Industrial Biotechnology,School of Biotechnology,Jiangnan University,Wuxi,214122,ChinaKey laboratory of Industrial Biotechnology,School of Biotechnology,Jiangnan University,Wuxi,214122,ChinaKey laboratory of Industrial Biotechnology,School of Biotechnology,Jiangnan University,Wuxi,214122,ChinaKey laboratory of Industrial Biotechnology,School of Biotechnology,Jiangnan University,Wuxi,214122,China
化学化工
Aldehyde dehydrogenaseNicotinamide cofactor biomimeticBiocatalysis
《Green Chemical Engineering》 2026 (3)
P.245-250,6
supported by the National Key R&D Program(2021YFC2102700)the National Natural Science Foundation of China(22377040,21907040)the support from the high-performance computing cluster platform of the School of Biotechnology,Jiangnan University。
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