Detection of tumor immunity-related ENPP1 using AIE-active probe QM-AMPOA
Ectonucleotide pyrophosphatase/phosphodiesterase 1(ENPP1),a crucial biomarker on the surface of tumor cells,plays a significant role in immune suppression and cancer metastasis.In this study,we successfully developed a novel amphiphilic fluorescent probe,QM-AMP,based on quinoline-malononitrile aggregation-induced emission(AIE)characteristics,for the real-time,and specific detection of ENPP1 enzymatic activity in multiple cell types.QM-AMP exhibits excellent dispersibility in both hydrophilic and hydrophobic environments,effectively minimizing background fluorescence interference and ensuring high sensitivity in ENPP1 detection.Upon interaction with ENPP1,the AMP group of the probe is specifically recognized and cleaved by ENPP1,which triggers the cleavage of the O–P bond and results in an“off-on”AIE fluorescence signal.QM-AMP demonstrates favorable photostability and biocompatibility,showing clear differential responses across cells with varying ENPP1 expression levels.Additionally,it can monitor ENPP1 expression changes under different macrophage polarization states,providing a potential tool for investigating ENPP1''s role in the tumor immune microenvironment.This study not only offers a new approach for in-situ detection of ENPP1 as a biomarker but also opens up new possibilities for early diagnosis and treatment of related diseases.
Yuwei Wang;Ting Yang;Shichang Liu;Haojian Yang;Qin Xu;Wei-Hong Zhu;Andong Shao;Qi Wang
Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering,Shanghai Key Laboratory of Functional Materials Chemistry,Frontiers Science Center for Materiobiology and Dynamic Chemistry,Institute of Fine Chemicals,School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai,200237,ChinaKey Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering,Shanghai Key Laboratory of Functional Materials Chemistry,Frontiers Science Center for Materiobiology and Dynamic Chemistry,Institute of Fine Chemicals,School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai,200237,ChinaKey Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering,Shanghai Key Laboratory of Functional Materials Chemistry,Frontiers Science Center for Materiobiology and Dynamic Chemistry,Institute of Fine Chemicals,School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai,200237,ChinaKey Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering,Shanghai Key Laboratory of Functional Materials Chemistry,Frontiers Science Center for Materiobiology and Dynamic Chemistry,Institute of Fine Chemicals,School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai,200237,ChinaKey Laboratory of Carbohydrate Vaccines and Drugs in Jiangsu Province,School of Life Sciences and Health Engineering,Jiangnan University,Wuxi 214122,ChinaKey Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering,Shanghai Key Laboratory of Functional Materials Chemistry,Frontiers Science Center for Materiobiology and Dynamic Chemistry,Institute of Fine Chemicals,School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai,200237,ChinaKey Laboratory of Carbohydrate Vaccines and Drugs in Jiangsu Province,School of Life Sciences and Health Engineering,Jiangnan University,Wuxi 214122,ChinaKey Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering,Shanghai Key Laboratory of Functional Materials Chemistry,Frontiers Science Center for Materiobiology and Dynamic Chemistry,Institute of Fine Chemicals,School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai,200237,China
医药卫生
ENPPAggregation-induced emissionAmphiphilic fluorescent probeMacrophage polarization
《Green Chemical Engineering》 2026 (4)
P.363-369,7
supported by the National Key Research and Development Program of China(2024YFA1803501)the NSFC Excellent Young Scientist(EYS)Scheme(22222803)the Basic Science Center of NSFC(T2488302)the Science and Technology Commission of Shanghai Municipality(24DX1400200)。
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