Dynamic exchange strategy for enzyme immobilization in Zr-based metal-organic frameworks for green synthesis of β-lactam antibioticsOA
Penicillin G acylase(PGA),a crucial biocatalyst in β-lactam antibiotic synthesis,has been significantly limited in its industrial application due to inherent stability issues.To address this critical challenge,efficient immobilization strategies and suitable carriers should be thoroughly investigated and developed.In this study,a novel dynamic exchange strategy was successfully implemented to immobilize PGA onto Zr-based metal-organic frameworks(Zr-MOFs),resulting in the fabrication of a series of PGA-enzyme composites(PGA@Zr-MOFs).These composites exhibited remarkable stability while retaining 61%–85% of the free enzyme activity.The PGA@ZrMOFs demonstrated exceptional catalytic performance in fixed-bed reactors under continuous operation conditions,maintaining 99% of their initial activity after 50 consecutive cycles.Furthermore,a cost-effective solidphase mechanical ball milling approach was developed for the synthesis of Zr-MOFs,which achieved comparable performance to previously mentioned materials.The versatility of this immobilization strategy was further demonstrated with oxidoreductases(formate dehydrogenase(FDH) and alcohol dehydrogenase(ADH)),which are essential enzymes in β-lactam antibiotic production.The immobilized oxidoreductases retained 51%–55% of their free enzyme activity and maintained 97% of their activity after 50 continuous cycles in fixed-bed operations.This strategy offers a promising platform for the eco-friendly and sustainable industrial production of β-lactam antibiotics.
Heng Hu;Shaochun Wu;Yunlong Zheng;Zhenjie Zhang;Mingfang Yang;Yao Chen
Key Laboratory of Biopharmaceutical Preparation and Delivery,State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing,100190,ChinaKey Laboratory of Biopharmaceutical Preparation and Delivery,State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing,100190,ChinaKey Laboratory of Biopharmaceutical Preparation and Delivery,State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing,100190,ChinaCollege of Chemistry,Nankai University,Tianjin,300071,ChinaKey Laboratory of Biopharmaceutical Preparation and Delivery,State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing,100190,ChinaKey Laboratory of Biopharmaceutical Preparation and Delivery,State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing,100190,China State Key Laboratory of Medicinal Chemical Biology,College of Pharmacy,Nankai University,Tianjin,300071,China Haihe Laboratory of Synthetic Biology,Tianjin,300308,China Frontiers Science Center for Cell Responses,Nankai University,Tianjin,300071,China
化学化工
Enzyme immobilizationMetal-organic frameworksDynamic exchange strategyEnzyme catalysisβ-Lactam antibiotic
《Green Chemical Engineering》 2026 (4)
P.389-398,10
supported by financial support from the National Key Research and Development Program of China (2021YFC2102100)Haihe Laboratory of Synthetic Biology (22HHSWSS00008)the National Natural Science Foundation of China (22371136)China Postdoctoral Science Foundation (General Program, 2022M721700)。
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