Characterization of MytiGal:a galactose‑binding lectin in the immune response of Mytilus coruscus–insights from sequence analysis,expression patterns,and bioactivitiesOA
Lectins are carbohydrate-binding proteins that function as pattern recognition receptors(PRRs)in animal innate immune system.Although invertebrates like mussels lack adaptive immune cells,they still mount pathogen-specific responses through rapidly recognition of pathogen-associated molecular patterns(PAMPs)by PRRs.Here,we identify and characterize a galactose-binding lectin from Mytilus coruscus,MytiGal,which belongs to the Mytilectin family.MytiGal features aβ-trefoil fold with conserved carbohydrate-binding sites and shares significant homology with lectins in other marine bivalves.It is highly expressed in the foot,mantle,and hemocytes,and its expression is significantly upregulated following bacterial challenge.Functional assays using recombinant MytiGal revealed its capacity to bind carbohydrates,broad-spectrum antimicrobial activity,and bacterial binding and agglutination activities.Additionally,MytiGal enhanced hemocyte phagocytic activity and facilitated bacterial clearance both in vivo and in vitro.It also alleviated bacteria-induced vacuolation and activated the antibacterial autophagy of hemocytes by regulating the autophagy pathway and the TLR/NFκB signaling pathway.These findings suggest that MytiGal plays a critical role in the immune response of mussels via the activation of antibacterial autophagy and provide new insights into the immune recognition mechanisms in marine bivalves.
Chuanyue Chen;Xueer Wang;Yingrong Jin;Yinchang Yao;Suchang Li;Zhi Liao;Xiaolin Zhang;Xiaojun Yan
Laboratory of Marine Biology Protein Engineering,Marine Science and Technical College,Zhejiang Ocean University,Zhoushan 316022,China School of Marine Sciences,Ningbo University,Ningbo 315211,ChinaLaboratory of Marine Biology Protein Engineering,Marine Science and Technical College,Zhejiang Ocean University,Zhoushan 316022,ChinaLaboratory of Marine Biology Protein Engineering,Marine Science and Technical College,Zhejiang Ocean University,Zhoushan 316022,ChinaLaboratory of Marine Biology Protein Engineering,Marine Science and Technical College,Zhejiang Ocean University,Zhoushan 316022,ChinaLaboratory of Marine Biology Protein Engineering,Marine Science and Technical College,Zhejiang Ocean University,Zhoushan 316022,ChinaLaboratory of Marine Biology Protein Engineering,Marine Science and Technical College,Zhejiang Ocean University,Zhoushan 316022,ChinaLaboratory of Marine Biology Protein Engineering,Marine Science and Technical College,Zhejiang Ocean University,Zhoushan 316022,ChinaLaboratory of Marine Biology Protein Engineering,Marine Science and Technical College,Zhejiang Ocean University,Zhoushan 316022,China School of Marine Sciences,Ningbo University,Ningbo 315211,China
农业科技
Mytilus coruscusGalactose-binding lectinMytiGalImmune responsePhagocytosisAntibacterial autophagy
《Marine Life Science & Technology》 2026 (2)
P.535-546,12
supported by the Natural Science Foundation of Zhejiang Province(LQ23D060002)National Natural Science Foundation of China(42020104009)National Key Research and Development Program of China(2023YFD2401902,2022YFD2401700).
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