Lycium Barbarum Polysaccharides Upregulate Trx2 in Schwann Cells through ESR1 to Repair Sciatic Nerve Injury in RatsOA

Objectives:Sciatic nerve injury(SNI)impairs quality of life,and Lycium barbarum polysaccharides(LBP)may exert therapeutic effects via regulating Schwann cell(SC)mitochondrial stability,though the mechanism remains unclear.The study aimed to elucidate the therapeutic mechanisms of LBP in mitigating sciatic nerve injury by protecting Schwann cells via the estrogen receptor 1(ESR1)/thioredoxin 2(Trx2)pathway.Methods:An in vitro SNI model was established by inducing RSC96 cells with H_(2)O_(2).Cell counting kit-8(CCK8)assay,enzyme-linked immunosorbent assay(ELISA),Western blot,reactive oxygen species(ROS)and adenosine triphosphate(ATP)quantification,and mitochondrial membrane potential(MMP)detection were used to evaluate cellular functions and molecular changes.Network pharmacology and molecular docking were employed to predict potential targets of LBP.Chromatin immunoprecipitation(ChIP)assays verified the interaction between ESR1 and Trx2,and ESR1 was knocked down in SCs to confirm the regulatory pathway.Additionally,in vivo validation was performed using a rat SNI model.Results:LBP intervention significantly reduced H_(2)O_(2)-induced mitochondrial dysfunction,inflammation,and oxidative stress in RSC96 cells(p<0.05).It also regulated the expression of apoptosis-related proteins,Trx2,and mitochondrial apoptosis-inducing factor(AIF;p<0.001).Network pharmacology and molecular docking identified ESR1 as a key target of LBP,and ChIP assays confirmed that ESR1 promoted Trx2 transcription.Moreover,LBP enhanced SC proliferation(OD_(450) at day 5=1.25-fold;colony formation=1.85-fold),increased MMP and ATP levels(MMP=2.25-fold;ATP=1.75-fold),inhibited inflammation,and reduced apoptosis(p<0.001),while ESR1 knockdown abrogated these effects(p<0.05).In rat SNI models,LBP effectively alleviated nerve injury(p<0.001).Conclusion:LBP may modulate Trx2 via ESR1 for the purpose of restoring the dysfunction of SCs induced by H_(2)O_(2).This potentially offers a new strategy for the therapy of SNI.