Vertebral morphological changes driven by rflna lead to body shape differentiation in carpOA
Body shape evolution in vertebrates frequently involves modifications in vertebral number or patterns of vertebral fusion,with distinct lineages displaying divergent trajectories.This study investigated the morphological and genetic basis of body shape variation between Hebao red carp(HB,Cyprinus carpio wuyuanensis)and Yellow River carp(YR,Cyprinus carpio haematopterus).Although both subspecies share an identical vertebral count(35),the compressed morphology of HB was attributable to skeletal anomalies,including vertebral shortening and fusion.Genome-wide association and population genetic analyses were performed on F1 and F2 hybrid cohorts to identify loci associated with this phenotype.A total of 231 selective sweep regions were detected across chromosomes A06,A08,A16,B05,and B06,with a prominent locus on chromosome A08(15.99-16.39 Mb)strongly correlated with body shape traits.Transcriptomic analysis revealed haplotype-dependent expression of rflna within this interval,implicating rflna in axial skeletal patterning.Functional validation using CRISPR/Cas9-mediated knockout of rfln in zebrafish(Danio rerio)induced vertebral malformations,including axial shortening,kyphosis,fusion,and a rounded abdominal profile.These results delineate the morphological and molecular framework governing axial remodeling in HB and highlight a conserved regulatory role for rflna in teleost skeletal development.
Zhou Jiang;Bi-Jun Li;Lin Chen;Ning Li;Shui-Mu Hu;Jing-Xin Ma;Bin-Ruo Wang;Fei Pu;Jian Xu;Yan-Hui Wang;Jian-Xin Feng;Xue-Jun Li;Tao Zhou;Peng Xu
State Key Laboratory of Mariculture Breeding,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,China Fujian Key Laboratory of Genetics and Breeding of Marine Organisms,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,ChinaState Key Laboratory of Mariculture Breeding,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,China Fujian Key Laboratory of Genetics and Breeding of Marine Organisms,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,ChinaState Key Laboratory of Mariculture Breeding,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,China Fujian Key Laboratory of Genetics and Breeding of Marine Organisms,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,China State Key Laboratory of Marine Environmental Science,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,ChinaState Key Laboratory of Mariculture Breeding,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,ChinaState Key Laboratory of Mariculture Breeding,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,ChinaState Key Laboratory of Mariculture Breeding,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,ChinaState Key Laboratory of Mariculture Breeding,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,ChinaState Key Laboratory of Mariculture Breeding,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,ChinaFisheries Engineering Institute,Chinese Academy of Fishery Sciences,Beijing 100141,ChinaHenan Academy of Fishery Sciences,Zhengzhou,Henan 450039,ChinaHenan Academy of Fishery Sciences,Zhengzhou,Henan 450039,ChinaCollege of Fisheries,Henan Normal University,Xinxiang,Henan 453007,ChinaState Key Laboratory of Mariculture Breeding,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,China Fujian Key Laboratory of Genetics and Breeding of Marine Organisms,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,ChinaState Key Laboratory of Mariculture Breeding,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,China Fujian Key Laboratory of Genetics and Breeding of Marine Organisms,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,China
农业科技
Body shapeHebao red carpYellow River carpSkeletal developmentrflna
《Zoological Research》 2026 (1)
P.128-138,11
supported by the National Natural Science Foundation of China(National Science Fund for Distinguished Young Scholars,National Science Fund for Excellent Young Scholars)(32225049,31422057)National Key R&D Program of China(2019YFE0119000)Postdoctoral Fellowship Program of CPSF(GZB20230386)Outstanding Postdoctoral Scholarship,State Key Laboratory of Marine Environmental Science at Xiamen University。
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