Clostridium butyricum mineralization of intracellular gold nanoclusters to boost biohydrogen production using visible lightOA
Photocatalytic intracellular biohybrid systems hold great potential for enhancing biohydrogen production using solar energy.However,conventional approaches face nanoparticle toxicity and inefficient transmembrane electron transfer.Here,we developed an intracellular biohybrid system via in-situ biomineralization of gold nanoclusters(Au NCs,2.15±0.68 nm)within C.butyricum.This biosynthesis leveraged native ion-transport proteins for Au(Ⅲ)uptake,synthesizing photocatalytically active Au NCs through cysteine-mediated reduction.This intracellular engineering confines photoexcitation and electron transfer within bacterial cells,eliminating transmembrane energy losses.As expected,the photoexcited Au NCs significantly enhanced intracellular NADH regeneration and ATP synthesis compared to dark controls,boosting metabolic reducing power and energy.This resulted in a 70.42%increase in hydrogen production(2.34 mol H_(2)·mol^(-1)glucose).Furthermore,Au NCs eliminated reactive oxygen species(ROS),improving system viability and stability under visible light.This work establishes a platform for engineering biocompatible hybrid systems via endogenous nanophotocatalyst self-assembly.
Yaoqiang Wang;Gang Xiao;Jianmin Xing;Haijia Su
State Key Laboratory of Green Biomanufacturing,Beijing Key Laboratory of Green Chemicals Biomanufacturing,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China CAS Key Laboratory of Green Process and Engineering&State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process,Ministry of Agriculture and Rural Affairs,Beijing 100193,ChinaState Key Laboratory of Green Biomanufacturing,Beijing Key Laboratory of Green Chemicals Biomanufacturing,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,ChinaCAS Key Laboratory of Green Process and Engineering&State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,ChinaState Key Laboratory of Green Biomanufacturing,Beijing Key Laboratory of Green Chemicals Biomanufacturing,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China
化学化工
Gold nanoclustersIn-situ biomineralizationIntracellular biohybrid systemsLight-driven hydrogen productionC.butyricum
《Green Energy & Environment》 2026 (3)
P.748-759,12
funded by the National Natural Science Foundation of China(22408378,22408019)the Fundamental Research Funds for the Central Universities(PY2517).
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