温阳活血方调节FOXA2/LIF/HOXA10通路增加子宫腺肌病在位内膜胞饮突数量的机制OA

Objective To investigate the effect of Wenyang Huoxue Formula on improving pinopodes in adenomyosis mice and its underlying mechanism.Methods A mouse model of adenomyosis was established using the tamoxifen method.Mice were divided into normal control group,model group,GnRH-α(gonadotropin-releasing hormone agonist)group,and low-,medium-,and high-dose Wenyang Huoxue Formula groups.Each group received the corresponding intervention for 8 weeks.The number of pinopodes was observed by scanning electron microscopy.The expression levels of Forkhead box A2(FOXA2),Leukemia Inhibitory Factor(LIF),and homeobox A10(HOXA10)in the pinopode formation pathway were detected by Western Blot and immunohistochemistry.The mRNA expression levels of FOXA2 and HOXA10 in the mouse endometrium were measured by q-PCR.Results Compared with the normal control group,no obvious pinopodes were observed in the endometrium of adenomyosis model mice(P＜0.05).The protein expression levels of FOXA2,LIF,and HOXA10 in the model group were significantly lower than those in the normal control group(P＜0.05),and the mRNA expression levels of FOXA2 and HOXA10 were also significantly decreased(P＜0.01).Compared with the model group,all doses(low,medium,and high)of Wenyang Huoxue Formula significantly increased the number of pinopodes in the endometrium of adenomyosis mice(P＜0.05).The high-dose Wenyang Huoxue Formula group significantly upregulated the protein expression levels of FOXA2,LIF,and HOXA10(P＜0.05)as well as the mRNA expression levels of FOXA2 and HOXA10(P＜0.01)compared with the model group.Furthermore,the effect of the high-dose Wenyang Huoxue Formula was similar to that of GnRH-α(P＞0.05).Conclusion Wenyang Huoxue Formula can activate the FOXA2/LIF/HOXA10 pathway to increase the number of pinopodes in the endometrium of adenomyosis mice,providing a mechanistic reference for improving endometrial receptivity in patients with adenomyosis-associated infertility.