甘蓝NAC基因家族鉴定及热胁迫下表达分析OA
Genome Wide Identification and Expression Analysis of the NAC Gene Family in Brassica oleracea under Heat Stress
NAC转录因子是植物中最大的基因家族之一,在植物生长发育及胁迫响应中发挥重要作用.为了解甘蓝NAC基因家族的特征及耐热调控机制,利用生物信息学方法对甘蓝NAC基因进行全基因组鉴定,并对其理化性质、染色体分布、基因结构、系统发育关系及启动子顺式作用元件进行系统分析;结合转录组数据和实时荧光定量PCR技术,检测甘蓝NAC基因在不同组织器官及高温处理下的表达模式;进一步利用本氏烟草叶片瞬时表达系统分析BoNAC36蛋白的亚细胞定位.结果表明,在甘蓝基因组中鉴定到204个BoNAC基因,编码氨基酸数量在154～855 aa之间,分子量为17752.17 Da～95180.96 Da,等电点介于4.33～9.83之间.204个BoNAC基因在9条染色体上呈不均匀分布,其中以5号染色体上的基因数目最多.根据拟南芥NAC蛋白的分类方法,BoNAC基因被分为16个亚家族,同一亚族内成员的保守基序和基因结构具有相似性.甘蓝NAC基因启动子区域包含多个光响应元件、激素应答元件、逆境胁迫响应元件及生长发育调控元件.组织表达分析发现,根中表达水平较高的BoNAC基因属于TIP和ATAF亚族.荧光定量PCR分析表明,14个BoNAC基因在热胁迫6 h和12 h持续高表达,其中BoNAC36的上调表达最明显.亚细胞定位结果显示,BoNAC36定位于细胞核.本研究为后续深入解析热胁迫下甘蓝NAC基因的功能提供重要的数据基础.
The NAC transcription factor is one of the largest gene families in plants,playing a crucial role in plant growth,development,and stress response.To elucidate the characteristics of the NAC gene family in cabbage and its regulatory mechanism underlying thermotolerance,this study employed bioinformatics methods to conduct a genome-wide identification of NAC genes in cabbage,and systematically analyzed their physicochemical properties,chromosomal distribution,gene structure,phylogenetic relationships,and promoter cis-acting elements.The expression patterns of cabbage NAC genes in different tissues and under high temperature stress treatment were examined by combining transcriptomic data and quantitative real-time PCR(qRT-PCR).Furthermore,the subcellular localization of the BoNAC36 protein was analyzed using a transient expression system in Nicotiana benthamiana leaves.The results showed that 204 BoNAC genes were identified in the cabbage genome,the number of encoded amino acids ranging from 154 to 855 aa,molecular weights ranging from 17752.17 Da to 95180.96 Da,and isoelectric points between 4.33 and 9.83.The 204 BoNAC genes were unevenly distributed across nine chromosomes,with the highest number of genes located on chromosome 5.Based on the classification method of Arabidopsis NAC protein,the BoNAC genes were divided into 16 subfamilies,with members within the same subfamily exhibiting similarities in conserved motifs and gene structures.The promoter regions of BoNAC genes contained multiple light-responsive elements,hormone-responsive elements,stress-responsive elements,and growth and development regulatory elements.Tissue expression analysis revealed that BoNAC genes with higher expression levels in roots belonged to the TIP and ATAF subfamilies.Quantitative real-time PCR analysis showed that 14 BoNAC genes were consistently and highly expressed at 6 h and 12 h under heat stress,among which BoNAC36 exhibited the most significant upregulated expression.Subcellular localization results indicated that BoNAC36 was localized in the nucleus.This study provides an important data foundation for further in-depth analysis of the function of NAC genes in cabbage under heat stress.
王敏;陈锦秀;薄天岳;杜子龙;顾大国;朱晓炜
上海市农业科学院园艺研究所/上海市设施园艺技术重点实验室,上海 201403上海市农业科学院园艺研究所/上海市设施园艺技术重点实验室,上海 201403上海市农业科学院园艺研究所/上海市设施园艺技术重点实验室,上海 201403上海市农业科学院园艺研究所/上海市设施园艺技术重点实验室,上海 201403上海星辉蔬菜有限公司,上海 201419上海市农业科学院园艺研究所/上海市设施园艺技术重点实验室,上海 201403
甘蓝NAC基因家族生物信息学表达分析亚细胞定位
cabbageNAC gene familybioinformaticsexpression analysissubcellular localization
《植物遗传资源学报》 2026 (6)
1292-1304,13
国家重点研发计划(2023YFD1201501)国家自然科学基金(32502709)上海市农业科学院卓越团队建设项目[农科卓2022(007)]National Key R&D Program of China(2023YFD1201501)National Natural Science Foundation of China(32502709)The Shanghai Academy of Agricultural Sciences Excellent Team Project[NongKeZhuo 2022(007)]
评论