复方赤石脂禹余粮散对小鼠止泻作用机制研究OA

The study explores the mechanism of anti-diarrheal effect of Compound Halloysitum Rubrum and Limonitum Powder in mice.The main element content was detected by ICP-OES,and the hygroscopicity was determined by the hygroscopic rate method.A total of 70 ICR mice were randomly divided into normal,model,Halloysitum Rubrum and Limonitum Decoction,Halloysitum Rubrum Powder,Limonitum Powder,Halloysitum Rubrum and Limonitum Powder,and positive control groups.Diarrhea models were prepared by gavage with castor oil,and the intestinal Evan's blue propulsion rate was measured.The pathological changes in the small and cecal intestinal wall tissues were observed,and the urea nitrogen,zinc ions,tumor necrosis factor-α(TNF-α)content,and Na+-K+ATPase activity were detected.Immunohistochemistry was used to detect the protein expression of interleukin-6(IL-6)and interleukin-1β(IL-1β),and 16S rRNA sequencing was performed on cecal contents.The results showed that the main elements of Halloysitum Rubrum and Limonitum Powder are Fe and Al,followed by Ca,Mn,Mg,Zn,etc.It significantly inhibited the intestinal Evan's blue propulsion rate in diarrheal mice,reduced intestinal wall cell damage and serum TNF-α content,increased intestinal wall cell Na+-K+ATPase activity and blood zinc ion concentration,and decreased the protein expression of IL-6 and IL-1β in intestinal wall tissues.It also reduced the relative abundance of Staphylococcus and Helicobacter while increasing the abundance of beneficial bacteria such as Lactobacillus,Faecalibacterium,and Bifidobacterium.Therefore,Halloysitum Rubrum and Limonitum Powder exerts its anti-diarrheal effect by supplementing trace elements,adsorbing inflammatory exudates,and regulating the balance of intestinal flora.